Establishment and characterization of NCC-MFS3-C1: a novel patient-derived cell line of myxofibrosarcoma
Establishment and characterization of NCC-MFS3-C1: a novel patient-derived cell line of myxofibrosarcoma
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NCC-MFS3-C1 的建立和表征：一种新型的患者来源的粘液纤维肉瘤细胞系
DOI：
10.1007/s13577-021-00548-6
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发表时间：
2021
期刊：
影响因子：
4.3
通讯作者：
Kondo Tadashi
中科院分区：
文献类型：
--
作者：
Tsuchiya Ryuto;Yoshimatsu Yuki;Noguchi Rei;Sin Yooksil;Ono Takuya;Sei Akane;Takeshita Fumitaka;Sugaya Jun;Iwata Shintaro;Yoshida Akihiko;Ohtori Seiji;Kawai Akira;Kondo Tadashi
黏液纤维肉瘤（MFS）是最具侵袭性的肉瘤之一，具有高度复杂的核型和基因组谱。虽然在治疗MFS时需要完全切除，但由于其强侵袭性，往往无法实现。此外，MFS对常规化疗难以耐受，预后较差。因此，有必要开发新的MFS治疗方式。患者来源的细胞系是基础研究和临床前研究的重要工具。然而，迄今为止仅报道了10个MFS细胞系。此外，在这些细胞系中，只有两种MFS细胞系是公开可用的。因此，我们利用从MFS患者手术切除的肿瘤标本，建立了一种新的MFS细胞系，命名为nc - mfs3 - c1。NCC-MFS3-C1细胞的拷贝数改变与原肿瘤相对应。NCC-MFS3-C1细胞表现为持续增殖、球状形成和侵袭性侵袭。在药物筛选试验中，蛋白酶体抑制剂硼替佐米和组蛋白去乙酰化酶抑制剂罗米地辛对nc - mfs3 - c1细胞表现出显著的抗增殖作用。因此，NCC-MFS3-C1细胞系是MFS基础研究和临床前研究的有用工具。
Myxofibrosarcoma (MFS) is one of the most aggressive sarcomas with highly complex karyotypes and genomic profiles. Although a complete resection is required in the treatment of MFS, it is often not achieved due to its strong invasive nature. Additionally, MFS is refractory to conventional chemotherapy, leading to poor prognosis. Therefore, it is necessary to develop novel treatment modalities for MFS. Patient-derived cell lines are important tools in basic research and preclinical studies. However, only 10 MFS cell lines have been reported to date. Furthermore, among these cell lines, merely two MFS cell lines are publicly available. Hence, we established a novel MFS cell line named NCC-MFS3-C1, using a surgically resected tumor specimen from a patient with MFS. NCC-MFS3-C1 cells had copy number alterations corresponding to the original tumor. NCC-MFS3-C1 cells demonstrate constant proliferation, spheroid formation, and aggressive invasion. In drug screening tests, the proteasome inhibitor bortezomib and the histone deacetylase inhibitor romidepsin demonstrated significant antiproliferative effects on NCC-MFS3-C1 cells. Thus, the NCC-MFS3-C1 cell line is a useful tool in both basic and preclinical studies for MFS.