Biotoxicity and bioavailability of hydrophobic organic compounds solubilized in nonionic surfactant micelle phase and cloud point system
Biotoxicity and bioavailability of hydrophobic organic compounds solubilized in nonionic surfactant micelle phase and cloud point system
复制标题
非离子表面活性剂胶束相和浊点体系中疏水性有机化合物的生物毒性和生物利用度
DOI：
10.1007/s11356-017-9076-4
复制
发表时间：
2017-06-01
影响因子：
5.8
通讯作者：
Dong, Wei
中科院分区：
文献类型：
--
作者：
Pan, Tao;Liu, Chunyan;Dong, Wei
最近的一项研究表明，溶解在某些非离子表面活性剂胶束相中的疏水性有机化合物对微生物表现出底物毒性，并增加了生物利用度。然而，在浊点系统中，可以防止生物毒性，因为化合物溶解成凝聚相，从而使一小部分化合物与细胞一起处于稀相。这项研究扩展了对溶解在非离子表面活性剂胶束相和浊点系统中的疏水性有机化合物的底物毒性和生物利用度之间关系的理解。在不同浓度的混合非离子表面活性剂Brij30和TMN-3存在下，以萘和菲进行生物毒性实验，它们在不同浓度下形成胶束相或浊点系统。酿酒酵母无法降解这些化合物，因此被用于生物毒性实验。浊点系统中的葡萄糖比非离子表面活性剂胶束相中的葡萄糖消耗得更快，表明溶解的化合物对非离子表面活性剂胶束相中的细胞的毒性增加。该结果通过后续的生物降解实验得到了验证。 PAH 降解细菌在浊点系统中比在胶束相中更快地降解化合物。所有这些结果表明，疏水性有机化合物的生物毒性随着表面活性剂胶束相中的生物利用度而增加，但在浊点系统中保持在较低水平。这些结果为浊点系统作为微生物转化或生物降解的新型介质的应用提供了指导。
A recent work has shown that hydrophobic organic compounds solubilized in the micelle phase of some nonionic surfactants present substrate toxicity to microorganisms with increasing bioavailability. However, in cloud point systems, biotoxicity is prevented, because the compounds are solubilized into a coacervate phase, thereby leaving a fraction of compounds with cells in a dilute phase. This study extends the understanding of the relationship between substrate toxicity and bioavailability of hydrophobic organic compounds solubilized in nonionic surfactant micelle phase and cloud point system. Biotoxicity experiments were conducted with naphthalene and phenanthrene in the presence of mixed nonionic surfactants Brij30 and TMN-3, which formed a micelle phase or cloud point system at different concentrations.Saccharomyces cerevisiae, unable to degrade these compounds, was used for the biotoxicity experiments. Glucose in the cloud point system was consumed faster than in the nonionic surfactant micelle phase, indicating that the solubilized compounds had increased toxicity to cells in the nonionic surfactant micelle phase. The results were verified by subsequent biodegradation experiments. The compounds were degraded faster by PAH-degrading bacterium in the cloud point system than in the micelle phase. All these results showed that biotoxicity of the hydrophobic organic compounds increases with bioavailability in the surfactant micelle phase but remains at a low level in the cloud point system. These results provide a guideline for the application of cloud point systems as novel media for microbial transformation or biodegradation.