Interactions between the thiol-group reagent N-ethylmaleimide and neutral and basic amino acid transporter-related amino acid transport
Interactions between the thiol-group reagent N-ethylmaleimide and neutral and basic amino acid transporter-related amino acid transport
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DOI：
10.1042/0264-6021:3430169
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发表时间：
1999-10-01
影响因子：
4.1
通讯作者：
Taylor, PM
中科院分区：
文献类型：
--
作者：
Peter, GJ;Davies, A;Taylor, PM
在肾和空肠刷状缘膜中表达的中性和碱性氨基酸转运蛋白（NBAT）参与氨基酸和胱氨酸的吸收。 NBAT 突变导致 1 型胱氨酸尿症。 C 端 myc 标记的 NBAT (NBATmyc) 在非洲爪蟾卵母细胞中表达时保留了 NBAT 的氨基酸转运和蛋白质-蛋白质相互作用特性。与卵母细胞中 NBATmyc 表达相关的中性氨基酸（Ala、Phe）-阳离子氨基酸（Arg）异质交换通过硫醇基试剂 N-乙基马来酰亚胺（NEM）处理而失活，但显着的 Arg-Arg 和 Ala-Ala 同质交换仍然存在。 NEM 异质交换活性的失活伴随着>85% 的丙氨酸和胱氨酸摄取的损失，且较小（
The neutral and basic amino acid transport protein (NBAT) expressed in renal and jejunal brush-border membranes is involved in amino acid and cystine absorption. NBAT mutations result in Type 1 cystinuria. A C-terminal myc-tagged NBAT (NBATmyc) retains the amino acid transport and protein-protein interaction properties of NBAT when expressed in Xenopus oocytes. Neutral amino acid (Ala, Phe)-cationic amino acid (Arg) heteroexchanges related to NBATmyc expression in oocytes are inactivated by treatment with the thiol-group reagent N-ethylmaleimide (NEM), although significant Arg-Arg and Ala-Ala homoexchanges persist. Inactivation of heteroexchange activity by NEM is accompanied by loss of >85% of alanine and cystine uptake, with smaller (