Separation of cisplatin and its hydrolysis products using electrospray ionization high-field asymmetric waveform ion mobility spectrometry coupled with ion trap mass spectrometry
Separation of cisplatin and its hydrolysis products using electrospray ionization high-field asymmetric waveform ion mobility spectrometry coupled with ion trap mass spectrometry
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DOI：
10.1021/ac0344182
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发表时间：
2003-11-01
影响因子：
7.4
通讯作者：
Mester, Z
中科院分区：
文献类型：
--
作者：
Cui, M;Ding, LY;Mester, Z
顺铂和它的单和二水合复合物已被分离使用高场不对称波形离子迁移谱（FAIMS）分析仪接口与电喷雾电离（ESI）和离子阱质谱（ITMS）。在FAIMS装置中向氮气幕/载气中添加氦气提高了电喷雾分析的灵敏度和选择性。引入三组分混合物作为帘幕/载气，氮气，氦气和二氧化碳，导致灵敏度进一步提高。与传统ESI-MS相比，ESI-FAIMS-ITMS光谱中的背景化学噪声显著降低，导致顺铂的信噪比提高了30倍以上。完整顺铂的分析结果在10-200 ng/mL浓度范围内呈线性，相应的检测限为0.7 ng/mL，分析前未进行衍生化或色谱分离。
Cisplatin and its mono- and dihydrated complexes have been separated using a high-field asymmetric waveform ion mobility spectrometry (FAIMS) analyzer interfaced with electrospray ionization (ESI) and ion trap mass spectrometry (ITMS). The addition of helium to the nitrogen curtain/carrier gas in the FAIMS device improved both the sensitivity and selectivity of the electrospray analysis. Introduction of a three-component mixture as curtain/carrier gas, nitrogen, helium, and carbon dioxide, resulted in further improvements to sensitivity. Compared with conventional ESI-MS, the background chemical noise in the ESI-FAIMS-ITMS spectrum was dramatically reduced, resulting in over 30-fold improvement in the signal-to-noise ratio for cisplatin. Analytical results were linear over the concentration range 10-200 ng/mL for intact cisplatin with a corresponding detection limit determined of 0.7 ng/mL with no derivatization or chromatographic separation prior to analysis.