The Genomic Landscape of the Somatic Linker Histone Subtypes H1.1 to H1.5 in Human Cells
The Genomic Landscape of the Somatic Linker Histone Subtypes H1.1 to H1.5 in Human Cells
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DOI：
10.1016/j.celrep.2013.05.003
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发表时间：
2013-06-01
期刊：
影响因子：
8.8
通讯作者：
Schneider, Robert
中科院分区：
文献类型：
--
作者：
Izzo, Annalisa;Kamieniarz-Gdula, Kinga;Schneider, Robert
人类细胞含有五种典型的复制依赖性体细胞组蛋白 H1 亚型（H1.1、H1.2、H1.3、H1.4 和 H1.5）。尽管它们是关键的染色质成分，但 H1 亚型的基因组分布仍然未知，并且它们在染色质过程中的作用迄今为止仍然难以捉摸。在这里，我们使用综合 DNA 腺嘌呤甲基转移酶识别 (DamID) 分析绘制了人肺成纤维细胞中所有体细胞复制依赖性 H1 亚型的基因组定位。我们发现，H1.2 至 H1.5 的 CpG 密集区和活性调控区普遍缺失。 H1.1 显示了与其他亚型不同的 DamID 结合谱，表明其具有独特的功能。 H1 亚型可以标记特定的域和抑制区域，表明 H1 在三维基因组组织中的作用。我们的工作将 H1 亚型整合到人类细胞的表观基因组图谱中，并为加深我们对 H1 及其异质性在基因组功能控制中的重要性的理解提供了宝贵的资源。
Human cells contain five canonical, replication-dependent somatic histone H1 subtypes (H1.1, H1.2, H1.3, H1.4, and H1.5). Although they are key chromatin components, the genomic distribution of the H1 subtypes is still unknown, and their role in chromatin processes has thus far remained elusive. Here, we map the genomic localization of all somatic replication-dependent H1 subtypes in human lung fibroblasts using an integrative DNA adenine methyltransferase identification (DamID) analysis. We find in general that H1.2 to H1.5 are depleted from CpG-dense regions and active regulatory regions. H1.1 shows a DamID binding profile distinct from the other subtypes, suggesting a unique function. H1 subtypes can mark specific domains and repressive regions, pointing toward a role for H1 in three-dimensional genome organization. Our work integrates H1 subtypes into the epigenome maps of human cells and provides a valuable resource to refine our understanding of the significance of H1 and its heterogeneity in the control of genome function.