Ethanol extract of Ganoderma lucidum ameliorates lipid metabolic disorders and modulates the gut microbiota composition in high-fat diet fed rats
Ethanol extract of Ganoderma lucidum ameliorates lipid metabolic disorders and modulates the gut microbiota composition in high-fat diet fed rats
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DOI：
10.1039/c8fo00836a
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发表时间：
2018-06-01
期刊：
影响因子：
6.1
通讯作者：
Lv, Xu-Cong
中科院分区：
文献类型：
--
作者：
Guo, Wei-Ling;Pan, Yu-Yang;Lv, Xu-Cong
本研究旨在探讨灵芝乙醇提取物（GL 95）对高脂饮食（HFD）大鼠血脂和肠道菌群的影响及其调控机制。UPLC-QTOF MS分析表明，GL 95富含三萜类化合物，特别是灵芝酸。动物实验结果表明，口服GL 95可显著降低血清总甘油三酯（TG）、总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）水平，抑制肝脏脂质蓄积和脂肪变性，从而明显改善血脂异常。此外，GL 95补充剂改变了肠道微生物群的组成，特别是与HFD组相比，调节了功能相关肠型的相对丰度。斯皮尔曼相关分析显示，Alistipes，Defluviitalea，消化球菌科和Alloprevotella与血清和肝脏脂质谱呈负相关。同时，GL 95处理调节参与脂质和胆固醇代谢的基因的mRNA表达水平。以上研究结果表明，灵芝三萜类化合物具有改善脂质代谢紊乱的潜力，部分是通过调节特定的肠道微生物群和调节参与脂质和胆固醇代谢的基因，表明灵芝三萜类化合物作为一种潜在的新型功能性食品用于治疗或预防血脂症。
The objective of this study was to investigate the effects of ethanol extract of Ganoderma lucidum (GL95) on hyperlipidaemia and gut microbiota, and its regulation mechanism in Wistar rats fed on a high-fat diet (HFD). UPLC-QTOF MS indicated that GL95 was enriched with triterpenoids, especially ganoderic acids. The results of the animal experiment showed that oral administration of GL95 markedly alleviated the dyslipidemia through decreasing the levels of serum total triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C), and inhibiting hepatic lipid accumulation and steatosis. Furthermore, GL95 supplementation altered the composition of gut microbiota, in particular modulating the relative abundance of functionally relevant enterotypes compared with the HFD group. The Spearman's correlation analysis revealed that Alistipes, Defluviitalea, Peptococcaceae and Alloprevotella were negatively correlated with serum and hepatic lipid profiles. Meanwhile, the GL95 treatment regulated the mRNA expression levels of the genes involved in lipid and cholesterol metabolism. The findings above illustrate that Ganoderma triterpenoids have the potential to ameliorate lipid metabolic disorders, in part through modulating specific gut microbiota and regulating the genes involved in lipid and cholesterol metabolism, suggesting Ganoderma triterpenoids as a potential novel functional food for the treatment or prevention of hyperlipidaemia.