Dengue tetravalent DNA vaccine inducing neutralizing antibody and anamnestic responses to four serotypes in mice
Dengue tetravalent DNA vaccine inducing neutralizing antibody and anamnestic responses to four serotypes in mice
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DOI：
10.1016/j.vaccine.2005.11.002
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发表时间：
2006-03-15
期刊：
影响因子：
5.5
通讯作者：
Imoto, JI
中科院分区：
文献类型：
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作者：
Konishi, E;Kosugi, S;Imoto, JI
我们开发了一种由表达四种血清型登革热病毒的前膜和包膜基因的质粒组成的登革热四价DNA疫苗。使用无针喷射注射器以100 μ g（每种血清型25 μ g）的剂量用四价疫苗免疫BALB/c小鼠两次，产生了针对所有血清型的中和抗体。该复方疫苗中包含的四种组分之间无干扰。四价疫苗免疫的小鼠在用每种登革热血清型攻击后表现出回忆性中和抗体应答：也诱导了对来自与用于中和试验的血清型不同的血清型的攻击的应答。(c)2005爱思唯尔有限公司保留所有权利。
We developed a dengue tetravalent DNA vaccine consisting of plasmids expressing premembrane and envelope genes of each of four serotypes of dengue viruses. BALB/c mice immunized twice with the tetravalent vaccine at a dose of 100 p,g (25 mu g for each serotype) using a needle-free jet injector developed neutralizing antibodies against all serotypes. There was no interference among the four components included in this combination vaccine. Tetravalent vaccine-immunized mice showed anamnestic neutralizing antibody responses following challenge with each dengue serotype: responses to challenges from serotypes different to those used for neutralization tests were also induced. (c) 2005 Elsevier Ltd. All rights reserved.