Impact of dietary sucralose and sucrose-sweetened water intake on lipid and glucose metabolism in male mice
Impact of dietary sucralose and sucrose-sweetened water intake on lipid and glucose metabolism in male mice
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DOI：
10.1007/s00394-022-02980-2
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发表时间：
2022-08
影响因子：
5
通讯作者：
Xinyi Wu;Le Cui;Haoquan Wang;Jinhong Xu;Zhaozhao Zhong;Xibei Jia;Jiaqi Wang;Huahua Zhang;Yanteng Shi;Yuhang Tang;Qianhui Yang;Qiongdan Liang;Yujing Zhang;Jing Li;Xiaohong Jiang
中科院分区：
文献类型：
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作者：
Xinyi Wu;Le Cui;Haoquan Wang;Jinhong Xu;Zhaozhao Zhong;Xibei Jia;Jiaqi Wang;Huahua Zhang;Yanteng Shi;Yuhang Tang;Qianhui Yang;Qiongdan Liang;Yujing Zhang;Jing Li;Xiaohong Jiang
过量饮用含糖饮料（SSB）与代谢紊乱的风险增加有关，包括肥胖和糖尿病。然而，越来越多的证据也表明，食用非营养性甜味剂（NNS）对体重和血糖控制有潜在的负面影响。最广泛使用的NNS三氯蔗糖的代谢作用仍然存在争议。本研究旨在比较膳食中摄入三氯蔗糖对（每日可接受摄入量，ADI剂量）和蔗糖糖水（在相同的甜度水平下）对雄性小鼠的脂质和葡萄糖代谢的影响。（0.1 mg/mL）或蔗糖（60 mg/mL）添加到8周龄雄性C57 BL/6小鼠的饮用水中16周，随后进行口服葡萄糖和腹膜内胰岛素耐量试验，以及骨矿物质密度、血浆脂质和激素的测量。小鼠被处死后，十二指肠和回肠被用于检查甜味受体（STR）和葡萄糖transporters.ResultsA显着增加的脂肪量的蔗糖组小鼠16周后，观察到甜饮水。蔗糖消耗还导致小鼠血浆LDL、胰岛素、肝脏脂质沉积水平升高，葡萄糖耐受不良增加。与蔗糖组相比，食用三氯蔗糖的小鼠表现出更低的脂肪积累、脂肪血症、肝脏脂肪变性和葡萄糖耐受不良。此外，每日剂量的三氯蔗糖只有一个温和的影响T1R2/3在肠道中，不影响葡萄糖转运蛋白和血浆胰岛素levels.ConclusionCompared与小鼠消耗蔗糖加糖的水，每日饮用三氯蔗糖的ADI剂量内有一个低得多的影响，对葡萄糖和脂质稳态。
AimsOverconsumption of sugar-sweetened beverages (SSBs) is associated with an increased risk of metabolic disorders, including obesity and diabetes. However, accumulating evidence also suggests the potential negative impact of consuming nonnutritive sweeteners (NNSs) on weight and glycaemic control. The metabolic effects of sucralose, the most widely used NNS, remain controversial. This study aimed to compare the impact of intake of dietary sucralose (acceptable daily intake dose, ADI dose) and sucrose-sweetened water (at the same sweetness level) on lipid and glucose metabolism in male mice.Materials and methodsSucralose (0.1 mg/mL) or sucrose (60 mg/mL) was added to the drinking water of 8-week-old male C57BL/6 mice for 16 weeks, followed by oral glucose and intraperitoneal insulin tolerance tests, and measurements of bone mineral density, plasma lipids, and hormones. After the mice were sacrificed, the duodenum and ileum were used for examination of sweet taste receptors (STRs) and glucose transporters.ResultsA significant increase in fat mass was observed in the sucrose group of mice after 16 weeks of sweetened water drinking. Sucrose consumption also led to increased levels of plasma LDL, insulin, lipid deposition in the liver, and increased glucose intolerance in mice. Compared with the sucrose group, mice consuming sucralose showed much lower fat accumulation, hyperlipidaemia, liver steatosis, and glucose intolerance. In addition, the daily dose of sucralose only had a moderate effect on T1R2/3 in the intestine, without affecting glucose transporters and plasma insulin levels.ConclusionCompared with mice consuming sucrose-sweetened water, daily drinking of sucralose within the ADI dose had a much lower impact on glucose and lipid homeostasis.