Genetic and metabolic biodiversity of Trichoderma from Colombia and adjacent neotropic regions
Genetic and metabolic biodiversity of Trichoderma from Colombia and adjacent neotropic regions
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DOI：
10.1016/j.fgb.2009.04.006
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发表时间：
2009-09-01
影响因子：
3
通讯作者：
Bissett, John
中科院分区：
文献类型：
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作者：
Hoyos-Carvajal, Lilliana;Orduz, Sergio;Bissett, John
木霉属已被研究用于生产酶和其他代谢物，以及用于开发作为有效的生物防治剂。木霉的生物多样性在新热带地区的研究相对有限。在当前的研究中，我们使用形态学、代谢和遗传方法评估了来自墨西哥、危地马拉、巴拿马、厄瓜多尔、秘鲁、巴西和哥伦比亚的183个分离株的生物多样性。物种多样性较高，包括29个分类群：棘孢木霉（60个分离株），深绿木霉（3），短密木霉（5），粗木霉（3），刺猬木霉（3），甘氏木霉（2），钩状木霉（2），哈茨木霉（49），拟康氏木霉（6），长臂木霉（3），卵孢木霉（1）、毛木霉（2）、罗斯木霉（4）、螺旋木霉（1）、绒毛木霉（3）、绿色木霉（8）、绿色木霉（7）和红褐肉座菌（3）（无性型：里氏木霉），沿着11个目前未描述的物种。T. Asperellum是优势种，由两种不同的基因型代表，具有不同的代谢谱和栖息地偏好。第二个优势种，哈茨木霉，由三个不同的基因型。增加11个目前未描述的物种是相当大的未解决的木霉在新热带地区的生物多样性的证据。核糖体重复序列的内部转录间隔区（ITS）的测序不能区分某些物种，单独使用时会产生几个错误识别，部分原因是在某些分离株中存在ITS的nonorthopathic拷贝。(c)2009年由Elsevier Inc.出版
The genus Trichoderma has been studied for production of enzymes and other metabolites, as well as for exploitation as effective biological control agents. The biodiversity of Trichoderma has seen relatively limited study over much of the neotropical region. In the current study we assess the biodiversity of 183 isolates from Mexico, Guatemala, Panama, Ecuador, Peru, Brazil and Colombia, using morphological, metabolic and genetic approaches. A comparatively high diversity of species was found, comprising 29 taxa: Trichoderma asperellum (60 isolates), Trichoderma atroviride (3), Trichoderma brevicompactum (5), Trichoderma crassum (3), Trichoderma erinaceum (3), Trichoderma gamsii (2), Trichoderma hamatum (2), Trichoderma harzianum (49), Trichoderma koningiopsis (6), Trichoderma longibrachiatum (3), Trichoderma ovalisporum (1), Trichoderma pubescens (2), Trichoderma rossicum (4), Trichoderma spirale (1), Trichoderma tomentosum (3), Trichoderma virens (8), Trichoderma viridescens (7) and Hypocrea jecorina (3) (anamorph: Trichoderma reesei), along with 11 currently undescribed species. T. asperellum was the prevalent species and was represented by two distinct genotypes with different metabolic profiles and habitat preferences. The second predominant species, T harzianum, was represented by three distinct genotypes. The addition of 11 currently undescribed species is evidence of the considerable unresolved biodiversity of Trichoderma in neotropical regions. Sequencing of the internal transcribed spacer regions (ITS) of the ribosomal repeat could not differentiate some species, and taken alone gave several misidentifications in part due to the presence of nonorthologous copies of the ITS in some isolates. (c) 2009 Published by Elsevier Inc.