Studies on glycosphingolipids of larvae of the green-bottle fly, Lucilia caesar. II. Isolation and structural studies of three glycosphingolipids with novel sugar sequences.
Studies on glycosphingolipids of larvae of the green-bottle fly, Lucilia caesar. II. Isolation and structural studies of three glycosphingolipids with novel sugar sequences.
复制标题
绿瓶蝇幼虫鞘糖脂的研究。
DOI：
10.1093/oxfordjournals.jbchem.a134002
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发表时间：
1982
影响因子：
2.7
通讯作者：
T. Hori
中科院分区：
文献类型：
--
作者：
M. Sugita;Y. Iwasaki;T. Hori
从绿瓶蝇Lucilia Caesar幼虫中分离到3种糖链上含有5~7个糖的新型鞘糖脂。经酶促降解、甲基化分析和部分酸解鉴定为：Gal-NACα(1-4)GalNAcβ(1-4)GlcNAcβ(1-3)Manβ(1-4)Glc-Cer，Galα(1-4)GalNAcα(1-4)GalNAcβ(1-4)GlcNAcβ(1-3)Manβ(1-4)Glc-Cer，和GlcNAcβ(1-4)Galα(1-4)Gal-Nacα(1-4)GalNAc beta(1-4)GlcNAc beta(1-3)Man beta(1-4)Glc-Cer。应当指出，它们的糖链中的一个N-乙酰半乳糖胺通过1-4键与另一个N-乙酰半乳糖胺以α-糖苷键连接，这是自然界中存在α(1-4)连接的半乳糖胺单元的第一个例子。主要脂肪酸为正饱和脂肪酸，碳数为16~22。长链碱基以C14和C16-4-鞘氨醇同系物为主。
Three novel glycosphingolipids containing five to seven sugars in their saccharide chains have been isolated from larvae of the green-bottle fly, Lucilia caesar. The structures of these glycolipids were identified by sequential enzymatic degradation with exoglycosidases, methylation analysis, and partial acid hydrolysis to be: Gal-NAc alpha (1-4)GalNAc beta (1-4)GlcNAc beta (1-3)Man beta (1-4)Glc-Cer, Gal alpha (1-4)GalNAc alpha (1-4)GalNAc beta (1-4)GlcNAc beta (1-3)Man beta (1-4)Glc-Cer, and GlcNAc beta (1-4)Gal alpha (1-4)Gal-NAc alpha (1-4)GalNAc beta (1-4)GlcNAc beta (1-3)Man beta (1-4)Glc-Cer. It should be noted that one N-acetylgalactosamine in their saccharide chains is alpha-glycosidically linked to another N-acetylgalactosamine by a 1-4 bond and that this is the first example of the presence of an alpha (1-4) linked galactosamine unit in nature. The major fatty acids were normal saturated fatty acids with carbon numbers of 16 to 22. The long-chain bases were dominated by C14 and C16-4-sphingenine homologues.