Specific antibody filter (SAF) binding capacity enhancement to remove anti-A antibodies.
Specific antibody filter (SAF) binding capacity enhancement to remove anti-A antibodies.
复制标题
特异性抗体过滤器 (SAF) 结合能力增强，可去除抗 A 抗体。
DOI：
10.1002/jbm.b.31707
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Federspiel,WilliamJ
中科院分区：
文献类型：
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作者：
Gautam,Shalini;Korchagina,ElenaY;Bovin,NicolaiV;Federspiel,WilliamJ
ABO血型不合移植前清除抗A/B抗体可预防超急性器官排斥反应。我们正在开发一种特异性抗体过滤器（SAF）装置，利用免疫亲和吸附从全血中选择性地去除ABO血型抗体。该器械由超滤中空纤维膜组成，该膜具有特异性结合固定在纤维内腔壁上的血型抗体的合成抗原。本研究的目的是评估抗原分子量和表面活化过程对增加纤维膜表面抗体结合能力的影响。与Atri-pNPA-30相比，新的更高分子量抗原Atri-pNSA-1000（A-三糖（Atri）与Mol.重量分别为1000 kDa和30 kDa）来改善抗原结合抗体的可及性。此外，溴化氰（CNBr）为基础的表面活化方法介导的TEA在中性pH介质中被用来提高抗原结合的活性位点的数量相比，强碱性介质的NaOH。使用CNBr/TEA活化法并通过固定化Atri-pNSA-1000抗原，在纤维表面上实现抗体结合容量（约0.01单克隆抗A IgM nmol/cm 2）。这种结合能力足以将单克隆抗体滴度从1：128降低到最终滴度低于1：4，表面积与体积比与商用透析装置相似（平均身体血液体积为5 L，表面积为1.1 m2）。© 2010 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，2010.
Removal of Anti‐A/B antibodies prior to ABO‐incompatible transplantation can prevent hyperacute organ rejection. We are developing a specific antibody filter (SAF) device to selectively remove ABO blood group antibodies from the whole blood by utilizing immunoaffinity adsorption. The device consists of ultrafiltration hollow fiber membranes with synthetic antigens specific to bind blood group antibodies immobilized on the inner lumenal walls of the fibers. The aim of this study was to evaluate the effect of antigen molecular weight and surface activation process to increase the antibody binding capacity of the fiber membrane surface. A new higher molecular weight antigen Atri‐pNSA‐1000 compared with Atri‐pNPA‐30 (A‐trisaccharide (Atri) conjugated to activated polymers of Mol. wt. 1000 kDa and 30 kDa, respectively) was employed to improve accessibility of the antigen to bind antibodies. Also, a cyanogen bromide (CNBr) based surface activation method mediated by TEA in neutral pH medium was used to enhance the number of active sites for antigen binding compared to a strong basic medium of NaOH. Using a CNBr/TEA activation method and by immobilizing Atri‐pNSA‐1000 antigen, an antibody binding capacity (∼0.01 monoclonal anti‐A IgM nmol/cm2) was achieved on the fiber surface. This binding capacity was sufficient to reduce monoclonal antibody titer from 1:128 to final titer below 1:4 with a surface area to volume ratio that is similar to commercial dialysis device (∼1.1 m2surface area for an average body blood volume of 5 L). © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.