Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance
Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance
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DOI：
10.1128/aac.00657-09
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发表时间：
2009-09-01
影响因子：
4.9
通讯作者：
Noverr, Mairi C.
中科院分区：
文献类型：
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作者：
Harriott, Melphine M.;Noverr, Mairi C.
白色念珠菌容易在留置医疗器械表面形成生物膜，这些生物膜是局部和全身感染的来源。据估计，27%的医院内C。白色念珠菌血流感染是多种微生物的，金黄色葡萄球菌是与C.白色念珠菌我们测试了S.金黄色葡萄球菌和C.白色念珠菌能够形成多微生物生物膜。虽然S.金黄色葡萄球菌在血清中形成的单一生物膜较差，在C.白色念珠菌在体系结构方面，S.金黄色葡萄球菌在生物膜表面形成小菌落，C.白色念珠菌作为底层支架。此外，S.金黄色葡萄球菌基质染色揭示了多微生物生物膜与单微生物生物膜的不同表型，表明S.金黄色葡萄球菌可被C.白色念珠菌S.金黄色葡萄球菌对万古霉素的耐药性在多微生物生物膜内增强，需要活的C. albicans，部分由C.白色念珠菌基质然而，C.白色念珠菌在多微生物生物膜中没有改变。
Candida albicans readily forms biofilms on the surface on indwelling medical devices, and these biofilms serve as a source of local and systemic infections. It is estimated that 27% of nosocomial C. albicans bloodstream infections are polymicrobial, with Staphylococcus aureus as the third most common organism isolated in conjunction with C. albicans. We tested whether S. aureus and C. albicans are able to form a polymicrobial biofilm. Although S. aureus formed poor monoculture biofilms in serum, it formed a substantial polymicrobial biofilm in the presence of C. albicans. In terms of architecture, S. aureus formed microcolonies on the surface of the biofilm, with C. albicans serving as the underlying scaffolding. In addition, S. aureus matrix staining revealed a different phenotype in polymicrobial versus monomicrobial biofilms, suggesting that S. aureus may become coated in the matrix secreted by C. albicans. S. aureus resistance to vancomycin was enhanced within the polymicrobial biofilm, required viable C. albicans, and was in part mediated by C. albicans matrix. However, the growth or sensitivity to amphotericin B of C. albicans is not altered in the polymicrobial biofilm.