Involvement of Cerebral Blood Flow on Neurological and Behavioral Functions after Subarachnoid Hemorrhage in Mice
Involvement of Cerebral Blood Flow on Neurological and Behavioral Functions after Subarachnoid Hemorrhage in Mice
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DOI：
10.1016/j.jstrokecerebrovasdis.2021.105952
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发表时间：
2021-06-29
影响因子：
2.5
通讯作者：
Hara, Hideaki
中科院分区：
文献类型：
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作者：
Matsubara, Hirofumi;Imai, Takahiko;Hara, Hideaki
目的：蛛网膜下腔出血（SAH）后的脑血流（CBF）变化与脑损伤（如早期脑损伤和迟发性脑缺血）密切相关。我们评估了SAH后使用激光散斑血流成像（LSFI）的CBF与亚急性期神经学检查结果之间的相关性。方法：采用血管内穿孔法建立雄性小鼠SAH模型。在6个时间点，立即到14天后SAH诱导使用LSFI定量测量CBF。评估行为测试和存活率。根据术后第1天的CBF将小鼠分为恢复组和低灌注组。结果：40只小鼠纳入本研究。低灌注组包括5只小鼠（20%），其余20只（80%）小鼠归为恢复组。恢复组脑血流量下降至术后1天。而低灌注组的CBF下降持续至术后7天。低灌注组的神经学表现和生存率明显差于恢复组。恢复组（n = 5）Y迷宫测试中低交替病例（< 50%）术后1天CBF明显降低。结论：蛛网膜下腔出血后第1天的低血流量与生存率、神经学表现和记忆障碍相关。脑血流的早期改善可能与SAH后预后的改善有关。
Objective: Cerebral Blood Flow (CBF) change after Subarachnoid Hemorrhage (SAH) is strongly associated with brain injuries such as early brain injury and delayed cerebral ischemia. We evaluated the correlation between CBF using Laser Speckle Flow Imaging (LSFI) after SAH and neurological findings in the sub-acute phase. Method: An SAH was induced by endovascular perforation in male mice. CBF was quantitatively measured by using LSFI at six time points, immediately to 14 days after SAH induction. Behavior tests and survival rate were evaluated. The mice were divided into recovery and hypo-perfusion groups according to their CBF at 1 day after the procedure. Result: Forty mice were included in this study. Five mice (20%) were included in the hypo-perfusion group, and the remaining 20 (80%) mice were classified as the recovery group. The decrease of CBF in the recovery group was observed until 1 day after the procedure. However, the decrease of CBF in the hypo-perfusion group was prolonged until 7 days after the procedure. Neurological findings and survival rates in the hypo-perfusion group were significantly worse than those in the recovery group. The low alternation cases (< 50%) in the Y maze test in the recovery group (n = 5) had significantly lower CBF at 1 day after the procedure. Conclusion: Low blood flow at 1 day after SAH was associated with worse survival rate, neurological findings, and memory disturbance. Early improvement in CBF may be associated with an improved prognosis after SAH.