The peritubular myoid cells in the testes from men with varicocele: an ultrastructural, immunohistochemical and quantitative study
The peritubular myoid cells in the testes from men with varicocele: an ultrastructural, immunohistochemical and quantitative study
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精索静脉曲张男性睾丸中的管周肌样细胞：超微结构、免疫组织化学和定量研究
DOI：
10.1111/j.1365-2559.1992.tb00426.x
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发表时间：
1992
期刊：
影响因子：
6.4
通讯作者：
Ricardo Paniagua
中科院分区：
文献类型：
--
作者：
L. Santamaría;R. Martín;Manuel Nistal;Ricardo Paniagua
本文研究了正常人和精索静脉曲张患者睾丸管周肌样细胞的超微结构和某些免疫表型特征。曲细精管分为五种类型（a-e），与固有层增厚测量的硬化进行性程度有关。在正常睾丸中只发现a型和B型小管，而精索静脉曲张患者的睾丸显示b-e型小管。a型和B型小管中的肌样细胞显示细长的胞质突起，具有丰富的平行排列的微丝束和电子致密体。在c小管中，肌样细胞显示相同的超微结构。晚期（d型）或完全（e型）硬化的肾小管肌样细胞显示出不规则的细胞核轮廓，很少的微丝束和缺乏电子致密体。抗肌动蛋白抗体对肌样细胞的免疫染色在a-c型小管中较强，在d型和e型小管中较弱。抗结蛋白抗体的免疫染色在a-d型小管中强烈，但c型和d型小管中的免疫反应细胞形状和分布不规则，e型小管中很少。抗波形蛋白抗体的免疫染色在a-c型小管中较弱，在d型和e型小管中较强。定量研究表明，随着硬化的进展，每个横截面小管中肌动蛋白和脱氨酶免疫反应细胞的数量以及这些细胞的免疫染色部分所占的表面积减少，而波形蛋白免疫反应细胞的数量及其免疫染色表面积增加。
Ultrastructural and some immunophenotypic features of the peritubular myoid cells of testes from normal men and from men with varicocele were studied. The seminiferous tubules were classified into five types (a–e), related to the progressive degree of sclerosis measured as thickening of the lamina propria. In normal testes only type a and b tubules were found, whereas the testes from men with varicocele showed type b–e tubules. Myoid cells in tubule types a and b showed slender cytoplasmic projections with abundant, parallel arranged microfilament bundles and electron‐dense bodies. In c tubules, the myoid cells showed the same ultrastructure. The myoid cells of tubules with advanced (type d) or complete (type e) sclerosis showed irregularly outlined nuclei, scant microfilament bundles and absence of electron‐dense bodies. Immunostaining of myoid cells with anti‐actin antibodies was intense in types a–c tubules and scant in types d and e. Immunostaining with anti‐desmin antibodies was intense in tubules types a–d, but the immunoreactive cells in types c and d tubules were irregularly shaped and distributed and were scanty in tubule type e. Immunostaining with anti‐vimentin antibodies was weak in types a–c tubules and intense in types d and e tubules. Quantitative studies revealed that, with the progression of sclerosis, the numbers of both actin‐ and desminimmunoreactive cells per cross‐sectioned tubule, and the surface area occupied by the immunostained portion of these cells, decreases while the number of vimentin‐immunoreactive cells and their immunostained surface area increases.