Early-Stage Dynamics in Vascular Endothelial Cells Exposed to Hydrostatic Pressure
Early-Stage Dynamics in Vascular Endothelial Cells Exposed to Hydrostatic Pressure
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DOI：
10.1115/1.4044046
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发表时间：
2019-09-01
影响因子：
1.7
通讯作者：
Sato, Masaaki
中科院分区：
文献类型：
--
作者：
Yoshino, Daisuke;Sato, Masaaki
血压是维持体内平衡和破坏体内平衡的重要因素。血管内皮细胞（EC）暴露于不同程度的血压，因此在这些生理和病理事件中发挥重要作用。然而，血压对EC功能的影响仍有待阐明。特别是，我们不知道EC如何感知和响应流体静压力的变化，即使流体静压力是已知的影响EC功能。在这里，我们假设，细胞的反应，导致报告的压力效应，发生在压力暴露的早期阶段，并观察早期阶段的动态EC阐明机制，通过EC的感觉和响应静水压力。我们发现，暴露于静水压力导致早期肌动球蛋白介导的收缩的内皮细胞没有改变细胞形态。这种反应可能是由暴露于静水压力后EC的水流出引起的。虽然只是一个有限的研究，这些发现确实解释了EC感知和响应静水压力的部分机制。
Blood pressure is an important factor both in maintaining body homeostasis and in its disruption. Vascular endothelial cells (ECs) are exposed to varying degrees of blood pressure and therefore play an important role in these physiological and pathological events. However, the effect of blood pressure on EC functions remains to be elucidated. In particular, we do not know how ECs sense and respond to changes in hydrostatic pressure even though the hydrostatic pressure is known to affect the EC functions. Here, we hypothesized that the cellular responses, leading to the reported pressure effects, occur at an early stage of pressure exposure and observed the early-stage dynamics in ECs to elucidate mechanisms through which ECs sense and respond to hydrostatic pressure. We found that exposure to hydrostatic pressure causes an early actomyosin-mediated contraction of ECs without a change in cell morphology. This response could be caused by water efflux from the ECs following exposure to hydrostatic pressure. Although only a limited study, these findings do explain a part of the mechanism through which ECs sense and respond to hydrostatic pressure.