Structure of FlgK reveals the divergence of the bacterial Hook-Filament Junction of Campylobacter
Structure of FlgK reveals the divergence of the bacterial Hook-Filament Junction of Campylobacter
复制标题
DOI：
10.1038/s41598-017-15837-0
复制
发表时间：
2017-11-16
影响因子：
4.6
通讯作者：
Samatey, Fadel A.
中科院分区：
文献类型：
--
作者：
Bulieris, Paula V.;Shaikh, Nausad H.;Samatey, Fadel A.
由多个部件组成的纳米机器的进化，每个部件都有特定的功能，是一个复杂的过程。如果要保持整体功能，一个部分的变化最终应导致其他部分的变化。在细菌鞭毛中，丝状体和钩状体具有不同的功能，它们各自的蛋白质FliC和FlgE具有不同的三维结构。灯丝起螺旋推进器的作用，钩起柔性万向节的作用。两种蛋白质FlgK和FlgL确保钩和细丝之间的平滑连接。在这里，我们表明，在弯曲杆菌，FlgK的3D结构不同于它的直向同源物在沙门氏菌和伯克霍尔德氏菌，其结构以前已经解决。将FlgK连接的模型对接到弯曲杆菌钩的结构上提供了一些关于其分歧的线索。这些数据表明，如何进化的压力，以适应结构的限制，由于弯曲杆菌钩的结构，导致发散的一个元素的超分子复合物，以保持整个鞭毛组件的功能。
Evolution of a nano-machine consisting of multiple parts, each with a specific function, is a complex process. A change in one part should eventually result in changes in other parts, if the overall function is to be conserved. In bacterial flagella, the filament and the hook have distinct functions and their respective proteins, FliC and FlgE, have different three-dimensional structures. The filament functions as a helical propeller and the hook as a flexible universal joint. Two proteins, FlgK and FlgL, assure a smooth connectivity between the hook and the filament. Here we show that, in Campylobacter, the 3D structure of FlgK differs from that of its orthologs in Salmonella and Burkholderia, whose structures have previously been solved. Docking the model of the FlgK junction onto the structure of the Campylobacter hook provides some clues about its divergence. These data suggest how evolutionary pressure to adapt to structural constraints, due to the structure of Campylobacter hook, causes divergence of one element of a supra-molecular complex in order to maintain the function of the entire flagellar assembly.