Possible role of azole and echinocandin lock solutions in the control of Candida biofilms associated with silicone
Possible role of azole and echinocandin lock solutions in the control of Candida biofilms associated with silicone
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DOI：
10.1016/j.ijantimicag.2010.12.016
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发表时间：
2011-04-01
影响因子：
10.8
通讯作者：
Imbert, Christine
中科院分区：
文献类型：
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作者：
Cateau, Estelle;Berjeaud, Jean-Marc;Imbert, Christine
到目前为止，与植入设备相关的念珠菌病的管理仍然存在问题。本研究的目的是研究体外抗白念珠菌和光滑念珠菌生物被膜的抗真菌锁定策略。用3种抗真菌药物对8株白色念珠菌和6株感染导管分离的光滑念珠菌进行了抗真菌试验。卡泊芬净和米卡芬净，棘球菌素，以及唑泊沙康唑进行了检测。采用100%硅胶导尿管上的念珠菌生物膜体外模型。分别在卡泊芬净(5 mg/L和25 mg/L)、米卡芬净(5 mg/L和15 mg/L)和泊沙康唑(10 mg/L)作用12h后12h和5d检测抗真菌锁对生物膜的杀灭效果，并在药物清除后1~3d观察抗菌膜活性的持久性。抗真菌锁被认为在生物膜酵母代谢活性显著下降的情况下是有效的(P<；0.001)。结果表明，米卡芬净对白念珠菌和光滑念珠菌生物被膜均有明显的抑制作用。此外，这一活动似乎持续了长达3天。卡泊芬净对所有白色念珠菌生物膜显示出相似的活性，但对光滑念珠菌生物膜的活性较差。泊沙康唑对白色念珠菌生物被膜的作用较弱，但其活性保持不变。棘球菌素锁定疗法可以通过对抗生物被膜和使设备能够就地维护，显著加强留置导尿管患者的念珠菌病的管理。(C)2011年爱思唯尔公司和国际化疗学会。版权所有。
Until now, management of candidiasis related to implanted devices has remained problematic. The aim of this study was to investigate antifungal lock strategies against Candida albicans and Candida glabrata biofilms in vitro. Three antifungal agents were used against eight C. albicans and six C. glabrata clinical strains isolated from infected catheters. Caspofungin and micafungin, both echinocandins, as well as the azole posaconazole were tested. An in vitro model of Candida biofilm on 100% silicone catheters was used. Efficacy of the antifungal lock was tested against biofilms aged 12 h and 5 days following exposure to caspofungin (5 mg/L and 25 mg/L), micafungin (5 mg/L and 15 mg/L) and posaconazole (10 mg/L) for 12 h. Persistence of antibiofilm activity was investigated 1-3 days following drug elimination. Antifungal lock was considered effective in the event of a significant decrease (P < 0.001) in the metabolic activity of the biofilm yeast. The results showed that micafungin had significant inhibitory effectiveness against young and mature C. albicans and C. glabrata biofilms. Moreover, this activity appeared to persist for up to 3 days. Caspofungin displayed similar activity against all C. albicans biofilms, but the activity was less persistent for C. glabrata biofilms. Posaconazole was less effective against C. albicans biofilms, but its activity was sustained. Echinocandin lock therapy could significantly enhance the management of candidiasis in patients with indwelling catheters by combating biofilms and enabling device maintenance in situ. (c) 2011 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.