First partial proteome of the poultry pathogen Mycoplasma synoviae
First partial proteome of the poultry pathogen Mycoplasma synoviae
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DOI：
10.1016/j.vetmic.2010.03.006
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发表时间：
2010-09-28
影响因子：
3.3
通讯作者：
Terenzi, Hernan
中科院分区：
文献类型：
--
作者：
Menegatti, Angela C. O.;Tavares, Carolina P.;Terenzi, Hernan
滑膜支原体对鸡和火鸡的呼吸道疾病和滑膜炎负有责任。为了鉴定滑膜分枝杆菌表达的最重要的蛋白质，采用双向凝胶电泳法和质谱仪相结合的方法绘制了滑膜分枝杆菌蛋白质组图谱。基于滑膜支原体基因组序列，利用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF)对30个不同的编码DNA序列进行了实验验证，其中包括一个假想的蛋白质和一个保守的假想蛋白质。根据蛋白质功能分类的同源类群对鉴定出的蛋白质进行分类。滑膜支原体有694个预测的CDS。总体而言，在这项工作中，考马斯亮蓝染色的2DE凝胶中的416个蛋白质点被解析，并用质谱仪(MS)进行分析。我们用MS共鉴定了78个蛋白质点，对应于30个不同的蛋白质。这是第一个在滑膜分枝杆菌中描述的蛋白质组图谱，有望作为比较分析的参考。(C)2010爱思唯尔B.V.保留所有权利。
Mycoplasma synoviae is responsible for respiratory tract disease and synovitis in chickens and turkeys. In an attempt to identify the most prominent proteins expressed by this microorganism, a proteome map of M. synoviae was developed by using two-dimensional gel electrophoresis in combination with mass spectrometry. Based on the genome sequence of M. synoviae, a total of 30 different coding DNA sequences, including one hypothetical and one conserved-hypothetical protein, were experimentally verified with the identification of the corresponding protein products by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). The identified proteins were assigned according to the Clusters of Orthologous Groups of proteins functional classification. M. synoviae has 694 predicted CDSs. Overall, in this work 416 proteins spots were resolved in Coomassie Blue stained 2DE gels and were analyzed by mass spectrometry (MS). Altogether, we have achieved by MS the identification of 78 protein spots, corresponding to 30 different proteins. This is the first proteome map to be described in M. synoviae, and it is expected to be useful as a reference for comparative analysis. (c) 2010 Elsevier B.V. All rights reserved.