Identification of a novel DNA molecule associated with Tobacco leaf curl virus
Identification of a novel DNA molecule associated with Tobacco leaf curl virus
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DOI：
10.1360/02tb9282
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发表时间：
2002-08-01
影响因子：
--
通讯作者：
Li, GX
中科院分区：
文献类型：
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作者：
Xie, Y;Zhou, XP;Li, GX
根据烟粉虱传双生病毒（WTG）的DNA β保守区序列设计引物，通过PCR方法，在感染烟草曲叶病毒（TLCV）Y 5和Y8分离物的烟草植株中鉴定出一个环状DNA分子，命名为DNA β。测定了Y 5和Y8的DNA β（TLCV DNA β）的全核苷酸序列。Y 5DNAbeta由1333个核苷酸组成，编码8个预测的开放阅读框，其中4个在病毒体正义DNA中，4个在互补正义DNA中; Y8 DNAbeta由1338个核苷酸组成，编码7个预测的开放阅读框，其中4个在病毒体正义DNA中，3个在互补正义DNA中。TLCV DNAbeta与TLCV DNA-A的序列同源性很低，除了它与TLCV DNA-A共享保守的TAATATTAC环序列。序列比较表明，Y 5和Y8 DNA β的同源性为85%，而Y 5和Y8 DNA β与已报道的胜红蓟黄脉病毒和棉花曲叶病毒DNA β的同源性较低（51%~ 65%）。免疫诱捕PCR和烟粉虱传毒试验表明，DNA β分子可被病毒颗粒包裹，并可被烟粉虱传播。这是国内首次报道的与风力发电机组相关的DNA β。
A circular DNA molecule, designated as DNAbeta, was identified in tobacco plants infected with,Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAbeta sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAbeta of Y5 and Y8 (TLCV DNAbeta) were determined. Y5 DNAbeta comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAbeta consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAbeta has little sequence homology to DNA-A of TLCV, except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAbeta shared 85% sequence homology with Y8 DNAbeta, and both Y5 DNAbeta and Y8 DNAbeta had relatively low sequence identity (51%-65%) with the reported DNAbeta molecules associated with Ageratum yellow vein virus and Cotton leaf curl virus. The immunotrapping PCR and whitefly transmission tests showed that DNAbeta molecule could be encapsidated in virus particle and transmitted by Bemisia tabaci. This is the first report of DNAbeta associated with WTGs in China.