Induction of the human sperm acrosome reaction with mannose-containing neoglycoprotein ligands.
Induction of the human sperm acrosome reaction with mannose-containing neoglycoprotein ligands.
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用含甘露糖的新糖蛋白配体诱导人精子顶体反应。
DOI：
10.1093/molehr/3.10.827
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发表时间：
1997
影响因子：
4
通讯作者：
Hershlag,A
中科院分区：
文献类型：
--
作者：
Benoff,S;Hurley,IR;Mandel,FS;Cooper,GW;Hershlag,A
在分类男性因素不育的情况下，我们特别注意人类精子表面的糖配体结合特性和顶体的胞吐功能能力-评估精子可再生性的关键参数。带识别和结合涉及精子表面甘露糖受体（凝集素）与透明带上甘露糖配体的相互作用。精子表面甘露糖凝集素可以通过其结合合成的模型配体异硫氰酸荧光素（FITC）缀合的甘露糖基化牛血清白蛋白（BSA）（Man-FITC-BSA）的能力来可视化。我们现在报告说，Man-FITC-BSA生物学上也模拟了溶解的真实的人类zonae的效果，在Man-FITC-BSA的结合导致时间依赖性受体聚集和诱导的顶体胞吐获能精子群体从生育供体。在我们的测定中，向Man-FITC-BSA中加入mM量的甘露糖单糖增加了由单个精子结合的多价甘露糖配体的数量，并增加了由Man-FITC-BSA诱导的顶体反应的速率，从而提高了标本处理效率。我们的结论是，暴露的人精子多价甘露糖配体+D-甘露糖单糖提供了一个新的，方便的和现成的系统来研究精子诱导顶体损失的能力。
In the interest of classifying cases of male factor infertility, we have paid particular attention to the sugar ligand binding properties of the human sperm surface and the functional capacity of the acrosome for exocytosis--key parameters for assessing sperm fertilizing ability. Zona recognition and binding involve the interactions of sperm surface mannose receptors (lectins) with mannose ligands on the zona pellucida. Sperm surface mannose lectins can be visualized by their ability to bind a synthetic model zona ligand, fluorescein isothiocyanate (FITC)-conjugated mannosylated bovine serum albumin (BSA) (Man-FITC-BSA). We now report that Man-FITC-BSA biologically also mimics the effects of solubilized authentic human zonae, in that binding of Man-FITC-BSA results in a time-dependent receptor aggregation and the induction of acrosome exocytosis in capacitated sperm populations from fertile donors. In our assay, the addition of mM amounts of mannose monosaccharide to Man-FITC-BSA increases the number of polyvalent mannose ligands bound by individual spermatozoa and increases the rate of the acrosome reactions induced by Man-FITC-BSA, thereby increasing specimen processing efficiency. We conclude that exposure of human spermatozoa to polyvalent mannose ligands + D-mannose monosaccharide offers a new, convenient and readily available system to study sperm capacity for induced acrosome loss.