Characterization of chimeric turnip yellow mosaic virus genomes that are infectious in the absence of aminoacylation.
Characterization of chimeric turnip yellow mosaic virus genomes that are infectious in the absence of aminoacylation.
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在没有氨酰化的情况下具有感染性的嵌合萝卜黄花叶病毒基因组的表征。
DOI：
10.1006/viro.1997.8475
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Dreher,TW
中科院分区：
文献类型：
--
作者：
Goodwin,JB;Skuzeski,JM;Dreher,TW
以前的实验已经鉴定了TYMC-TMVPSK嵌合基因组，其中芜菁黄花叶病毒(TYMV)的3‘-tRNA结构被烟草花叶病毒的3’-tRNA序列取代。该基因组在萝卜原生质体中的积累水平约为野生型的3%，但在植物上不具侵染性。在本研究中，将TYMV序列引入该嵌合体3‘区域的反密码子环和氨基酸受体臂中，导致病毒积累增加三到四倍。两个这样修改的嵌合基因组在植物中引起了稳定的感染。TYMC-XX和TYMC-YY病毒在植物中传代后，在3‘末端积累了微小的序列变化，具有很强的侵染性。根据外壳蛋白水平，原生质体中病毒的积累量约为野生型的40%，植物中病毒粒子的产量约为0.1 mg/g叶片。广泛的实验未能在体外检测到这些基因组RNA的氨基酰化，但它们是小麦胚CCA核苷酸转移酶的活性底物。在单独的实验中，TYMV RNA的3‘-tRNA样结构被丹参潜伏的TymoVirus RNA的3’端96个核苷酸取代，产生了一个对植物具有侵染性的基因组(分离株TYMC-H)。这种嵌合病毒在植物中产生的症状和病毒粒子产量与TYMC-XX和-YY相似，尽管原生质体中外壳蛋白的积累是野生型的13%。病毒RNA不是CCA核苷酸转移酶的底物，不能被氨基酰化。TYMC-XX、-YY和-H是已知的第一个TYMV复制子，可以在没有氨基酰化的情况下有效地扩增并感染植物。它们的活性表明，其他性质可以弥补氨基酰化的缺失。
Previous experiments have characterized the chimeric genome TYMC-TMVPSK, in which the 3′-tRNA-like structure of turnip yellow mosaic virus (TYMV) was replaced by 3′ sequences from tobacco mosaic virus. This genome accumulated in turnip protoplasts to a level about 3% of wild type, but was not infectious on plants. In the present study, TYMV sequences introduced into the anticodon loop and amino acid acceptor arm of the 3′ region of this chimera led to three- to fourfold increases in viral accumulation. Two such modified chimeric genomes gave rise to stable infections in plants. After further passaging in plants and the accumulation of minor sequence changes in the 3′ terminal region, the resultant viruses, TYMC-XX and TYMC-YY, were highly infectious. Viral accumulations in protoplasts were about 40% of wild type on the basis of coat protein levels, and virion yields in plants were about 0.1 mg/g leaf. Extensive assays failed to detect aminoacylation of these genomic RNAsin vitro,but they were active substrates for wheat germ CCA nucleotidyltransferase. In separate experiments, the 3′-tRNA-like structure of TYMV RNA was replaced by the 3′ terminal 96 nucleotides from erysimum latent tymovirus RNA, resulting in a genome that was infectious to plants (isolate TYMC-H). This chimeric virus produced similar symptoms and virion yield in plants as TYMC-XX and -YY, although accumulations of coat protein in protoplasts were 13% of wild type. The viral RNA was a poor substrate for CCA nucleotidyltransferase and could not be aminoacylated. TYMC-XX, -YY, and -H are the first TYMV replicons known to amplify efficiently and infect plants in the absence of aminoacylation. Their viability suggests that other properties can compensate for the absence of aminoacylation.
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影响因子：
10.5
作者：
GALLIE, DR;WALBOT, V
通讯作者：
WALBOT, V
对来自萝卜黄花叶病毒 RNA 的克隆 cDNA 的 tRNA 样转录物进行的验证表明，病毒 RNA 3 端的 L 形区域不足以实现最佳氨酰化。
影响因子：
3.9
作者：
T. Dreher;C. Florentz;R. Giegé
通讯作者：
R. Giegé
RNA植物病毒的繁殖和纯化
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
L. Lane
通讯作者：
L. Lane
携带复制酶基因和3非编码区异源组合的嵌合烟草花叶病毒的复制。
影响因子：
3.7
作者：
M. Ishikawa;T. Meshi;Y. Watanabe;Y. Okada
通讯作者：
Y. Okada
第一个植物tRNAHis的一级结构
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
M. Barciszewska;G. Keith;G. Dirheimer;J. Barciszewski
通讯作者：
J. Barciszewski