Benchmarks of Biomembrane Force Probe Spring Constant Models.
Benchmarks of Biomembrane Force Probe Spring Constant Models.
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DOI：
10.1016/j.bpj.2017.10.013
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发表时间：
2017-12
影响因子：
3.4
通讯作者：
L. Ju;C. Zhu
中科院分区：
文献类型：
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作者：
L. Ju;C. Zhu
由于其柔软的弹性常数、与活细胞的兼容性和用户友好的可操作性，生物膜力探头(BFP)作为一种表征活细胞(1，2)上单分子动力学的皮力技术显示了其独特的强度。最近的同步荧光成像(3-5)和双BFP设置(6)的升级进一步增加了生物物理学和机械生物学领域对BFP的认识，将其作为一种强大的纳米工具来可视化活细胞表面的分子机械传感事件(7)。BFP使用微吸管吸入的红细胞(RBC)，其顶端连接着一个玻璃珠作为力传感器。这种力传感器的弹簧常数的确定是BFP技术的基础，因为这是力测量的基础。将BFP近似为Hookean弹簧，Evans等人在生物物理杂志上发表了三个用于计算BFP弹簧常数的解析表达式。在1995(8)中，Simson et al.在1998(9)中，和Heinrich等人。2007年(10)。虽然这三个表达式都是从RBC力学中推导出来的，但在建立数学问题时做了不同的简化假设，并使用不同的近似来求解数学问题，从而引入了不同的误差量。在这一简短的交流中，我们对已发表的三个bfp弹性常数的解析表达式进行了基准测试。我们发现，它们的准确性取决于BFP的配置(图1)。要考虑的BFP弹性常数(Kp)的三个解析表达式是Kp？PDP
Thanks to its soft spring constant, compatibility with live cells, and user-friendly maneuverability, the biomembrane force probe (BFP) demonstrates its unique strength as a picoforce technique for characterizing single-molecule dynamics on live cells (1, 2). The recent upgrades with simultaneously fluorescence imaging (3–5) and dual BFP setup (6) have further increased the recognition of the BFP by biophysics and mechanobiology fields as a powerful nanotool to visualize molecular mechanosensing events on live cell surface (7). The BFP uses a micropipette-aspirated red blood cell (RBC) with a glass bead attached to its apex as a force transducer. The spring constant determination of this force transducer is fundamental to the BFP technique as this is the basis for the force measurement. Approximating the BFP as a Hookean spring, three analytical expressions have been published in Biophysical Journal for calculation of the BFP spring constant by Evans et al. in 1995 (8), Simson et al. in 1998 (9), and Heinrich et al. in 2007 (10). While all three expressions were derived from RBC mechanics, different simplifying assumptions were made to set up the mathematical problems and different approximations were used to solve the mathematical problems, which introduce different amount of errors. In this brief communication, we benchmarked the three published analytical expressions of the BFP spring constant. We found that their accuracies depend on BFP configurations (Fig. 1). The three analytical expressions of BFP spring constant (kp) to be considered are kp ¼ pDp