Identification of novel cAMP responsive element modulator (CREM) isoforms expressed by osteoblasts.
Identification of novel cAMP responsive element modulator (CREM) isoforms expressed by osteoblasts.
复制标题
成骨细胞表达的新型 cAMP 反应元件调节剂 (CREM) 亚型的鉴定。
DOI：
10.1007/s00223-005-0003-1
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Kream,BE
中科院分区：
文献类型：
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作者：
Liu,F;Huang,Y-F;Kream,BE
CREM是环腺苷单磷酸（cAMP）响应元件调节剂，属于cAMP响应转录因子的多基因家族。CREM通过利用四个启动子和一个复杂的替代信使核糖核酸剪接模式编码多种不同的异构体。我们之前的研究表明，甲状旁腺激素在成骨细胞中诱导CREM P2启动子产物ICER（诱导型cAMP早期抑制因子）。在此，我们报道成骨细胞也表达至少15个由P1启动子启动的CREM转录本，包括7个由选择性剪接产生的新转录本。令人感兴趣的是，我们发现CREM-X同时包含之前仅在P3启动子产物中发现的外显子θ1和位于外显子θ1上游的新外显子L。
CREM, the cyclic adenosine monophosphate (cAMP) responsive element modulator, belongs to a multigene family of cAMP-responsive transcription factors. CREM encodes a variety of different isoforms by utilizing four promoters and a complex pattern of alternative messenger ribonucleic acid (mRNA) splicing. We showed previously that parathyroid hormone induces the CREM P2 promoter products known as ICER (inducible cAMP early repressor) in osteoblasts. Herein we report that osteoblasts also express at least 15 CREM transcripts initiated from the P1 promoter, including 7 novel transcripts that result from alternative splicing. It is of interest that we found that CREM-X contains both exon θ1, previously identified only in P3 promoter products, and a new exon termed L, which is located upstream of exon θ1.