Bioluminescence Imaging to Track Bacteroides fragilis Inhibition of Vibrio parahaemolyticus Infection in Mice.
Bioluminescence Imaging to Track Bacteroides fragilis Inhibition of Vibrio parahaemolyticus Infection in Mice.
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DOI：
10.3389/fcimb.2017.00170
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发表时间：
2017
影响因子：
5.7
通讯作者：
Zhi F
中科院分区：
文献类型：
--
作者：
Li Z;Deng H;Zhou Y;Tan Y;Wang X;Han Y;Liu Y;Wang Y;Yang R;Bi Y;Zhi F
脆弱拟杆菌是人体肠道的一种厌氧、革兰氏阴性共生细菌。它在促进免疫系统成熟和抑制异常炎症方面起着重要作用。近年来许多研究都集中在脆弱芽孢杆菌与人体免疫的关系上，并表明脆弱芽孢杆菌具有许多有益的益生菌作用。由于对肠道病原菌的抑制作用是益生菌菌株的重要特征，本研究考察了脆弱芽孢杆菌是否能抑制致病菌。结果表明，脆弱芽孢杆菌对体外培养的副溶血性弧菌有抑制作用，且脆弱芽孢杆菌对LoVo细胞和RAW 264.7细胞均有保护作用。利用体内成像技术，我们构建了一株发光的副溶血性弧菌菌株，并在小鼠模型中发现脆弱芽孢杆菌可以缩短定植时间，减少luxx表达菌的数量。这些结果为将脆弱芽孢杆菌开发成益生菌产品提供了有用的信息，也表明这种共生菌可能有助于副溶血性芽孢杆菌引起的胃肠炎的临床治疗。
Bacteroides fragilis is an anaerobic, Gram-negative, commensal bacterium of the human gut. It plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. Many recent studies have focused on the relationship between B. fragilis and human immunity, and indicate that B. fragilis has many useful probiotic effects. As inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether B. fragilis could inhibit pathogenic bacteria. Results showed that Vibrio parahaemolyticus was inhibited by B. fragilis in vitro, and that B. fragilis could protect both RAW 264.7 and LoVo cells from damage caused by V. parahaemolyticus. Using in vivo imaging, we constructed a light-emitting V. parahaemolyticus strain and showed that B. fragilis might shorten the colonization time and reduce the number of lux-expressing bacteria in a mouse model. These results provide useful information for developing B. fragilis into a probiotic product, and also indicate that this commensal bacterium might aid in the clinical treatment of gastroenteritis caused by V. parahaemolyticus.