Agarose-dextran gels as synthetic analogs of glomerular basement membrane: Water permeability
Agarose-dextran gels as synthetic analogs of glomerular basement membrane: Water permeability
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DOI：
10.1016/s0006-3495(02)75555-0
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发表时间：
2002-04-01
影响因子：
3.4
通讯作者：
Deen, WM
中科院分区：
文献类型：
--
作者：
White, JA;Deen, WM
合成了新型琼脂糖-葡聚糖水凝胶，并通过测量其达西（水力）渗透性（κ）来检查其作为肾小球基底膜实验模型的适用性。通过电子束照射实现了大葡聚糖分子在琼脂糖中的固定化。使用两种clextran分子量（500或2000）中的任一种，用琼脂糖体积分数（0.04或0.08中的0）和clextran体积分数（phi（d））（纤维体积/凝胶体积）在0至0.02范围内制备复合凝胶。在任一琼脂糖浓度和任一大小的clextran，K显着下降的clextran的量增加。对于非常小体积分数的葡聚糖，获得了与纯琼脂糖的K值的统计学显著偏差：对于phi（a）= 0.04，phi（d）大于或等于0.0003;对于phi（a）=-0.08，phi（d）大于或等于0.001。达西渗透性对phi（d）比对phi（a）更敏感，并且比纯琼脂糖的达西渗透性小26倍。虽然（p）是一个重要的变量，但clextran分子量不是。使用简单的结构理想化，葡聚糖添加对K的影响被描述得相当好。在高琼脂糖浓度下，clextran链表现为散布在粗琼脂糖原纤维之间的细纤维，而在低浓度下，clextran分子开始类似于嵌入琼脂糖凝胶中的球形障碍物。利用这些材料实现生理学相关的达西渗透性（低至1.6nm（2））的能力使它们成为肾小球基底膜和可能的其他细胞外基质的有吸引力的实验模型。
Novel agarose-dextran hydrogels were synthesized and their suitability as experimental models of glomerular basement membrane was examined by measuring their Darcy (hydraulic) permeabilities (kappa). Immobilization of large dextran molecules in agarose was achieved by electron beam irradiation. Composite gels were made with agarose volume fractions (0 of 0.04 or 0.08 and clextran volume fractions (phi(d)) ranging from 0 to 0.02 (fiber volume/gel volume), using either of two clextran molecular weights (500 or 2000). At either agarose concentration and for either size of clextran, K decreased markedly as the amount of clextran was increased. Statistically significant deviations from the value Of K for pure agarose were obtained for remarkably small volume fractions of dextran: phi(d) greater than or equal to 0.0003 for phi(a) = 0.04 and phi(d) greater than or equal to 0.001 for phi(a) = -0.08. The Darcy permeabilities were much more sensitive to phi(d) than to phi(a), and were as much as 26 times smaller than those of pure agarose. Although (p, was an important variable, clextran molecular weight was not. The effects of dextran addition on K were described fairly well using simple structural idealizations. At high agarose concentrations, the clextran chains behaved as fine fibers interspersed among coarse agarose fibrils, whereas, at low concentrations, the clextran molecules began to resemble spherical obstacles embedded in agarose gels. The ability to achieve physiologically relevant Darcy permeabilities with these materials (as low as 1.6 nm(2)) makes them an attractive experimental model for glomerular basement membrane and possibly other extracellular matrices.