TRIM31 is downregulated in non-small cell lung cancer and serves as a potential tumor suppressor
TRIM31 is downregulated in non-small cell lung cancer and serves as a potential tumor suppressor
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DOI：
10.1007/s13277-014-1763-x
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发表时间：
2014-06-01
期刊：
影响因子：
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通讯作者：
He, Ping
中科院分区：
文献类型：
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作者：
Li, Hui;Zhang, Yi;He, Ping
本研究旨在探讨TRIM31在人非小细胞肺癌(NSCLC)中的表达模式及其生物学作用。应用免疫组织化学方法检测了116例非小细胞肺癌组织和20例相应正常肺组织中TRIM31的表达。我们发现40.5%(47/116)的癌组织中TRIM31表达下调，这与肿瘤状态(p=0.0132)、分期(p=0.001)和有无淋巴结转移(p=0.0382)有关。TRIM31在肺癌细胞系中的表达低于正常支气管细胞系HBE。将TRIM31基因导入H157和H1299细胞。TRIM31过表达抑制了两株细胞的生长速度和集落形成能力。此外，细胞周期调节因子细胞周期蛋白D_1和细胞周期蛋白E的表达在TRIM31转染后明显降低。综上所述，TRIM31可能是非小细胞肺癌的一种肿瘤抑制因子。
The present study aims to investigate expression pattern and biological roles of TRIM31 in human non-small cell lung cancer (NSCLC). We examined TRIM31 expression in 116 NSCLC tissues and 20 corresponding normal lung tissues by immumohistochemistry. We found TRIM31 downregulation in 47 out of 116 (40.5 %) cancer samples, which correlated with tumor status (p = 0.0132), advanced p-TNM stage (p = 0.001), and nodal metastasis (p = 0.0382). TRIM31 expression was lower in lung cancer cell lines than normal bronchial cell line HBE. Transfection of TRIM31 plasmid was performed in H157 and H1299 cells. TRIM31 overexpression inhibited cell growth rate and colony formation ability in both cell lines. In addition, expression of cell cycle regulator cyclin D1 and cyclin E were decreased after TRIM31 transfection. In conclusion, TRIM31 might serve as a tumor suppressor in non-small cell lung cancer.