Cholesterol-to-phospholipid ratio in whole sperm and seminal plasma from fertile stallions and stallions with unexplained subfertility
Cholesterol-to-phospholipid ratio in whole sperm and seminal plasma from fertile stallions and stallions with unexplained subfertility
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DOI：
10.1016/j.anireprosci.2006.03.018
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发表时间：
2007-05-01
影响因子：
2.2
通讯作者：
Varner, D. D.
中科院分区：
文献类型：
--
作者：
Brinsko, S. P.;Love, C. C.;Varner, D. D.
精液样本来自6匹有生育能力的种马和7匹患有不明原因不育的种马。活动精子百分率(77.5+/-11.3vs67.5+/-12.2，P=0.2)和渐进性活动精子百分率(70.8+/-13.6vs60.7+/-14.0，P=0.2)相似。对照公马和患病公马精液的形态特征(%正常：60.2vs52.9+/-111.3。P=0.4；异常头数百分比7.3+/-4.8对12.1+/-5.0，P=0.11；与钙离子载体A23187孵育后，能育种马精子顶体反应率为96+/-2.8%，而原因不明不育种马精子顶体反应率仅为2.9+/-2.5%(P<；0.001)。在能生育和不能生育的种马之间，整个精子和精浆中胆固醇和磷脂的摩尔量没有差别(P>；0.1)。而雄马精浆中胆固醇与磷脂的摩尔比是可育种马的2.5倍(P=0.09)和1.9倍(P=0.009)。(C)2006爱思唯尔B.V.保留所有权利。
Semen samples were collected from six fertile stallions and seven stallions with unexplained infertility. Percentages of motile sperm (77.5 +/- 11.3 versus 67.5 +/- 12.2, P = 0.2), and progressively motile sperm (70.8 +/- 13.6 versus 60.7 +/- 14.0, P = 0.2) were similar between fertile and subfertile stallions, respectively. Morphologic characteristics in ejaculates of control and affected stallions (% normal: 60.2 +/- 18.2 versus 52.9 +/- 111.3. P = 0.4; % abnormal heads 7.3 +/- 4.8 versus 12.1 +/- 5.0, P = 0.11; and % abnormal acrosomes 1.6 +/- 2.1 versus 3.0 +/- 3.4, P = 0.4) did not differ. After incubation with the calcium ionophore A23187, acrosome reaction rate of sperm from fertile stallions was 96 +/- 2.8% whereas only 2.9 +/- 2.5% of sperm from stallions with unexplained subfertility had acrosome reacted (P < 0.001). Molar amounts of cholesterol and phospholipid in whole sperm and seminal plasma did not differ (P > 0.1) between fertile and subfertile stallions. However, the molar ratio of cholesterol-to-phospholipid was 2.5 times greater in the seminal plasma (P = 0.09) and 1.9 times greater (P = 0.009) in whole sperm of subfertile stallions compared to fertile stallions. (c) 2006 Elsevier B.V. All rights reserved.