Synthesis and evaluation of novel biodegradable hydrogels based on poly(ethylene glycol) and sebacic acid as tissue engineering scaffolds
Synthesis and evaluation of novel biodegradable hydrogels based on poly(ethylene glycol) and sebacic acid as tissue engineering scaffolds
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DOI：
10.1021/bm700924n
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发表时间：
2008-01-01
影响因子：
6.2
通讯作者：
Lu, Lichun
中科院分区：
文献类型：
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作者：
Kim, Jinku;Lee, Kee-Won;Lu, Lichun
合成了一种新型的可生物降解聚乙二醇（PEG）基水凝胶，即PEG癸二酸二丙烯酸酯（PEGSDA），并对其性能进行了评价。这些聚合物的化学结构通过傅里叶变换红外光谱和质子核磁共振（H-1 NMR）光谱进行了确认。光聚合后，动态剪切模量的水凝胶是高达0.2 MPa的50%PEGSDA水凝胶，显着高于传统的水凝胶，如PEG二丙烯酸酯（PEGDA）。这些大分子单体的溶胀比显著低于PEGDA。体外降解研究表明，这些水凝胶是可生物降解的，在37 ℃下在磷酸盐缓冲盐水中孵育8周后，25%和50%PEGSDA的重量损失约为66%和32%。在体外生物相容性进行了评估，使用培养的大鼠骨髓基质细胞（MSC）在未反应的单体或降解产物的存在下。与传统的PEGDA水凝胶不同，没有RGD肽修饰的PEGSDA水凝胶诱导MSC细胞粘附类似于组织培养聚苯乙烯。最后，采用固体自由成型技术制备了复杂三维结构的PEGSDA水凝胶，其结构完整性比PEGDA水凝胶更好地保持。这些水凝胶可用作组织工程应用的支架。
Novel biodegradable poly(ethylene glycol) (PEG) based hydrogels, namely, PEG sebacate diacrylate (PEGSDA) were synthesized, and their properties were evaluated. Chemical structures of these polymers were confirmed by Fourier transform infrared and proton nuclear magnetic resonance (H-1 NMR) spectroscopy. After photopolymerization, the dynamic shear modulus of the hydrogels was up to 0.2 MPa for 50% PEGSDA hydrogel, significantly higher than conventional hydrogels such as PEG diacrylate (PEGDA). The swelling ratios of these macromers were significantly lower than PEGDA. The in vitro degradation study demonstrated that these hydrogels were biodegradable with weight losses about 66% and 32% for 25% and 50% PEGSDA after 8 weeks of incubation in phosphate-buffered saline at 37 degrees C. In vitro biocompatibility was assessed using cultured rat bone marrow stromal cells (MSCs) in the presence of unreacted monomers or degradation products. Unlike conventional PEGDA hydrogels, PEGSDA hydrogel without RGD peptide modification induced MSC cell adhesion similar to tissue culture polystyrene. Finally, complex three-dimensional structures of PEGSDA hydrogels using solid free form technique were fabricated and their structure integrity was better maintained than PEGDA hydrogels. These hydrogels may find use as scaffolds for tissue engineering applications.