P2X7 receptor of rat dorsal root ganglia is involved in the effect of moxibustion on visceral hyperalgesia
P2X7 receptor of rat dorsal root ganglia is involved in the effect of moxibustion on visceral hyperalgesia
复制标题
大鼠背根神经节P2X(7)受体参与艾灸对内脏痛觉过敏的影响
DOI：
10.1007/s11302-014-9439-y
复制
发表时间：
2015-06-01
影响因子：
3.5
通讯作者：
Liang, Shangdong
中科院分区：
文献类型：
--
作者：
Liu, Shuangmei;Shi, Qingming;Liang, Shangdong
肠易激综合征（IBS）和炎症性肠病通常表现出内脏过敏。炎症刺激后内脏伤害感受器产生传入神经冲动，通过背根神经节（DRG）传输到中枢神经系统。 ATP 及其激活的嘌呤能 2X(7) (P2X(7)) 受体在伤害性信号的传递中发挥重要作用。嘌呤能信号传导参与内脏疼痛的感觉传递。艾灸是用点燃的艾草直接或间接作用于穴位或身体其他特定部位来治疗疾病的疗法。热敏穴是疾病状态下对艾热极其敏感的对应穴位。本研究旨在探讨艾灸热敏穴位“大肠俞”的镇痛作用与结直肠扩张慢性炎症刺激后大鼠背根神经节P2X(7)受体表达水平的关系。热敏灸大肠舒穴位通过降低内脏痛引起的DRG中P2X(7)mRNA和蛋白的上调表达水平来抑制伤害性信号传递,并逆转DRG中胶质纤维酸性蛋白(GFAP,卫星胶质细胞的标志物)的异常表达。因此，内脏疼痛模型中的腹部撤回反射（AWR）评分降低，疼痛阈值升高。因此，热敏灸大肠舒穴位可以通过抑制上调的P2X(7)受体介导的伤害性传导来对IBS产生治疗作用。
Irritable bowel syndrome (IBS) and inflammatory bowel disease often display visceral hypersensitivity. Visceral nociceptors after inflammatory stimulation generate afferent nerve impulses through dorsal root ganglia (DRG) transmitting to the central nervous system. ATP and its activated-purinergic 2X(7) (P2X(7)) receptor play an important role in the transmission of nociceptive signal. Purinergic signaling is involved in the sensory transmission of visceral pain. Moxibustion is a therapy applying ignited mugwort directly or indirectly at acupuncture points or other specific parts of the body to treat diseases. Heat-sensitive acupoints are the corresponding points extremely sensitive to moxa heat in disease conditions. In this study, we aimed to investigate the relationship between the analgesic effect of moxibustion on a heat-sensitive acupoint "Dachangshu" and the expression levels of P2X(7) receptor in rat DRG after chronic inflammatory stimulation of colorectal distension. Heat-sensitive moxibustion at Dachangshu acupoint inhibited the nociceptive signal transmission by decreasing the upregulated expression levels of P2X(7) mRNA and protein in DRG induced by visceral pain, and reversed the abnormal expression of glial fibrillary acidic protein (GFAP, a marker of satellite glial cells) in DRG. Consequently, abdominal withdrawal reflex (AWR) score in a visceral pain model was reduced, and the pain threshold was elevated. Therefore, heat-sensitive moxibustion at Dachangshu acupoint can produce a therapeutic effect on IBS via inhibiting the nociceptive transmission mediated by upregulated P2X(7) receptor.