Dynamic functional imaging of relative cerebral blood volume during rat forepaw stimulation
Dynamic functional imaging of relative cerebral blood volume during rat forepaw stimulation
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DOI：
10.1002/mrm.1910390415
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发表时间：
1998-04-01
影响因子：
3.3
通讯作者：
Weisskoff, RM
中科院分区：
文献类型：
--
作者：
Mandeville, JB;Marota, JJA;Weisskoff, RM
在高碳酸血症和局灶性神经元激活的大鼠模型中，在注射具有很长血液半衰期的血管内造影剂后，使用T-2加权成像进行脑血容量（CBV）的区域变化的动态测量。高碳酸血症期间计算的CBV变化百分比与其他非侵入性方式的文献结果一致。使用自旋回波和梯度回波图像发现CBV增加的百分比相等，表明毛细血管和小静脉的血容量成比例变化。在大鼠前爪的电刺激期间，在刺激开始和停止后，相对于血氧水平依赖（BOLD）信号，局部CBV对刺激的反应（24 +/-4%）显著延迟。刺激后CBV衰减与BOLD刺激后下冲在时间上一致。相对于BOLD成像，外源性试剂的使用在2特斯拉的磁场强度下将功能性对比噪声比增加了5.7 +/- 1.3，在4.7特斯拉的磁场强度下增加了1.5 +/- 0.2。
Dynamic measurements of regional changes in cerebral blood volume (CBV) were performed in rat models of hypercarbia and focal neuronal activation using T-2-weighted imaging after injection of an intravascular contrast agent with a very long blood half-life. Calculated percent CBV change during hypercarbia was consistent with literature results from other noninvasive modalities. Equivalent percent CBV increases were found using spin-and gradient-echo images, suggesting proportional changes in blood volume for capillaries and small veins. During electrical stimulation of rat forepaw, focal CBV response to stimulation (24 +/- 4%) was significantly delayed relative to blood oxygen level dependent (BOLD) signal after both onset and cessation of stimulation. Poststimulus CBV decay was temporally consistent with the BOLD poststimulus undershoot. The use of exogenous agent increased the functional contrast-to-noise ratio relative to BOLD imaging by 5.7 +/- 1.3 at a magnetic field strength of 2 Tesla and 1.5 +/- 0.2 at 4.7 Tesla.