A hydroxychalcone derived from cinnamon functions as a mimetic for insulin in 3T3-L1 adipocytes
A hydroxychalcone derived from cinnamon functions as a mimetic for insulin in 3T3-L1 adipocytes
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DOI：
10.1080/07315724.2001.10719053
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发表时间：
2001-08-01
影响因子：
3.5
通讯作者：
Graves, DJ
中科院分区：
文献类型：
--
作者：
Jarvill-Taylor, KJ;Anderson, RA;Graves, DJ
目的：这些研究调查的能力，从肉桂的羟基查耳酮作为胰岛素模拟物在3 T3-L1 adipocyte.Methods：比较实验进行了肉桂甲基羟基查耳酮聚合物和胰岛素方面的葡萄糖摄取，糖原合成，磷脂酰肌醇-3-激酶依赖性，糖原合成酶激活和糖原合成酶激酶-3 β活性。结果：MHCP处理刺激葡萄糖摄取和糖原合成的胰岛素类似的水平。糖原的合成被抑制渥曼青霉素和LY 294002，抑制剂直接对PI-3-激酶。此外，MHCP处理激活糖原合酶并抑制糖原合酶激酶-3 β活性，这是胰岛素处理的已知效应。胰岛素受体的分析表明，该受体在暴露于MHCP时被磷酸化。这支持胰岛素级联是由MHCP触发的。沿着将MHCP与胰岛素进行比较，用MHCP和胰岛素组合进行实验。使用双重治疗观察到的反应大于添加剂，表明两个compounds.Conclusion之间的协同作用：总之，这些结果表明，MHCP是一种有效的胰岛素模拟物。MHCP可用于治疗胰岛素抵抗和研究导致细胞中葡萄糖利用的途径。
Objectives: These studies investigated the ability of a hydroxychalcone from cinnamon to function as an insulin mimetic in 3T3-L1 adipocytes.Methods: Comparative experiments were performed with the cinnamon methylhydroxychalcone polymer and insulin with regard to glucose uptake, glycogen synthesis, phosphatidylinositol-3-kinase dependency, glycogen synthase activation and glycogen synthase kinase-3 beta activity. The phosphorylation state of the insulin receptor was also investigated.Results: MHCP treatment stimulated glucose uptake and glycogen synthesis to a similar level as insulin. Glycogen synthesis was inhibited by both wortmannin and LY294002, inhibitors directed against the PI-3-kinase. In addition, MHCP treatment activated glycogen synthase and inhibited glycogen synthase kinase-3 beta activities, known effects of insulin treatment. Analysis of the insulin receptor demonstrated that the receptor was phosphorylated upon exposure to the MHCP. This supports that the insulin cascade was triggered by MHCP. Along with comparing MHCP to insulin, experiments were done with MHCP and insulin combined. The responses observed using the dual treatment were greater than additive, indicating synergism between the two compounds.Conclusion: Together, these results demonstrate that the MHCP is an effective mimetic of insulin. MHCP may be useful in the treatment of insulin resistance and in the study of the pathways leading to glucose utilization in cells.