Sustained hydrogen photoproduction by phosphorus-deprived Chlamydomonas reinhardtii cultures
Sustained hydrogen photoproduction by phosphorus-deprived Chlamydomonas reinhardtii cultures
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DOI：
10.1016/j.ijhydene.2012.01.068
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发表时间：
2012-05-01
影响因子：
7.2
通讯作者：
Kosourov, Sergey N.
中科院分区：
文献类型：
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作者：
Batyrova, Khorcheska A.;Tsygankov, Anatoly A.;Kosourov, Sergey N.
本研究表明，除硫剥夺外，在缺磷(p)条件下培养莱茵衣藻还可以产生持续的H-2光。然而，藻类在磷培养基中再悬浮不能使藻类细胞缺磷，这显然是由于细胞中有大量的磷储备。在本研究中，通过将洗涤后的藻类接种到低初始细胞密度（低于2mg Chl(-1)）的-P培养基中来实现缺磷。在初始生长阶段，藻类利用胞内磷，建立厌氧环境，随后进入H-2光合作用阶段。在初始Chl含量为1 mg l（-1）的培养基中，H-2的最大输出量为70 ml l（-1）。Chl大于2 mg l（-1）的培养不产生H-2气体。藻类培养物对磷缺乏的生理反应与藻类对硫缺乏的生理反应具有显著的相似性。版权所有(C) 2012，氢能源出版有限责任公司由爱思唯尔有限公司出版版权所有。
This study demonstrates that, besides sulfur deprivation, sustained H-2 photoproduction in Chlamydomonas reinhardtii cultures can be generated by incubating algae under phosphorus-deprived (-P) conditions. However, phosphorus deficiency in algal cells could not be obtained by resuspension of algae in -P medium, evidently due to a significant reserve of phosphorus in cells. In this study, phosphorus deficiency was accomplished by inoculating the washed algae into the -P medium at low initial cell densities (below 2 mg Chl l(-1)). After the initial growth period, where cells utilize intracellular phosphorus, algae established anaerobic environment followed by the period of H-2 photoproduction. The maximum H-2 output (similar to 70 ml l(-1)) was obtained in cultures with the initial Chl content similar to 1 mg l(-1). Cultures with Chl above 2 mg l(-1) did not produce H-2 gas. The physiological response of algal cultures to phosphorus deprivation demonstrated significant similarities with the response of algae to sulfur depletion. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.