MICROBIOLOGICAL SYNTHESIS OF L-TYROSINE AND 3,4-DIHYDROXYPHENYL-L-ALANINE .1. DISTRIBUTION OF TYROSINE PHENOL LYASE IN MICROORGANISMS
MICROBIOLOGICAL SYNTHESIS OF L-TYROSINE AND 3,4-DIHYDROXYPHENYL-L-ALANINE .1. DISTRIBUTION OF TYROSINE PHENOL LYASE IN MICROORGANISMS
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DOI：
10.1080/00021369.1972.10860505
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发表时间：
1972-01-01
期刊：
影响因子：
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通讯作者：
MATSUI, H
中科院分区：
文献类型：
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作者：
ENEI, H;OKUMURA, S;MATSUI, H
本文研究了酪氨酸酚裂解酶活性在微生物中的分布，用完整的细胞在含有l-丝氨酸和苯酚或邻苯二酚的合成反应混合物中。在各种细菌中发现了这种活性，其中大多数属于肠杆菌科，尤其是埃希氏菌属、变形杆菌属和欧文氏菌属。从培养24小时的发酵液中制备的Erwinia acetolaATCC 21434完整细胞具有显著的高酶活性，并能催化1-酪氨酸或3-羟色胺的合成反应，4-二羟苯基-L-丙氨酸（L-多巴）产物的分离和鉴定结果表明，用这种酶法合成的氨基酸，与左旋酪氨酸或左旋多巴相同。
The distribution of tyrosine phenol lyase activity in microorganisms was studied with intact cells in a synthetic reaction mixture containingl-serine and phenol or pyrocatechol. This activity was found in various bacteria, most of which belonged to theEnterobacteriaceae; especially to the generaEscherichia, ProteusandErwinia. Cells ofErwinia herbicolaATCC 21434 were selected as a promising source of enzyme.Intact cells ofErwinia herbicolaATCC 21434 prepared from a broth cultured for 24 hr contained markedly high enzymic activity and catalyzed the synthetic reaction ofl-tyrosine or 3,4-dihydroxyphenyl-l-alanine (l-dopa) froml-serine and phenol or pyrocatechol in significantly high yields.Results of the isolation and identification of the products showed that the amino acid synthesized by this enzymatic method was identical withl-tyrosine orl-dopa.