Liquid Chromatographic Analysis of Adriamycin and Metabolites in Biological Fluids
Liquid Chromatographic Analysis of Adriamycin and Metabolites in Biological Fluids
复制标题
生物体液中阿霉素及其代谢物的液相色谱分析
DOI：
10.1080/01483917808060035
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发表时间：
1978
影响因子：
1.3
通讯作者：
M. Garnick
中科院分区：
文献类型：
--
作者：
M. Israel;W. Pegg;P. Wilkinson;M. Garnick
摘要本文描述了一种使用两种互补的高效液相色谱系统（一种正相和另一种反相）分析血浆、胆汁和尿液中广泛使用的抗肿瘤药物阿霉素水平的确定性方法。通过荧光检测实现每个样品2-10皮摩尔范围内的灵敏度。平行使用两个系统提供了一个明确的基础，表征和测定阿霉素及其主要代谢产物，阿霉素，并使蒽环类荧光苷元和共轭物的测量，以及。
Abstract A definitive approach to the analysis of plasma, bile, and urine levels of the widely used antitumor drug Adriamycin using two complementary high performance liquid chromatographic systems, one normal phase and the other reversed phase, is described. Sensitivity in the 2–10 picomole per sample range is achieved by means of fluorescence detection. The use of the two systems in parallel provides an unequivocal basis for the characterization and determination of Adriamycin and its principal metabolite, adriamycinol, and enables the measurement of anthracycline fluorescent aglycones and conjugates, as well.