Molecular Spectroscopic Studies of Farrerol Interaction with Calf Thymus DNA
Molecular Spectroscopic Studies of Farrerol Interaction with Calf Thymus DNA
复制标题
Farrerol 与小牛胸腺 DNA 相互作用的分子光谱研究
DOI：
10.1021/jf2019006
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发表时间：
2011-08-24
影响因子：
6.1
通讯作者：
Hu, Mingming
中科院分区：
文献类型：
--
作者：
Zhang, Guowen;Fu, Peng;Hu, Mingming
在pH 7.4的Tris-HCl缓冲溶液中，以中性红（NR）染料为探针，采用紫外-可见吸收光谱、荧光光谱、圆二色谱（CD）、粘度测定和DNA熔解技术研究了杜鹃素与小牛胸腺DNA的相互作用。结果表明，杜鹃素分子可嵌入DNA碱基对中，碘离子猝灭效应和单链DNA（ssDNA）猝灭效应减弱，诱导CD光谱变化，DNA相对粘度和变性温度显著升高。采用交替最小二乘法（ALS）对杜鹃素和NR与DNA竞争反应的光谱数据矩阵进行了解析，得到了杜鹃素、NR和DNA-NR复合物的浓度分布和相应的纯光谱。该ALS分析证明了通过取代DNA-NR中的NR，杜鹃醇嵌入DNA。复杂.通过van 't霍夫方程计算了杜鹃素与DNA的结合焓变（Δ H度）和熵变（Δ S度）分别为-16.49 +/-051 kJ mol（-1）和32.47 +/-1.02 J mol（-1）K-1，表明杜鹃素与DNA的结合主要是由疏水作用和氢键驱动的.
The interaction between farrerol and calf thymus DNA in a pH 7.4 Tris-HCl buffer was investigated with the use of neutral red (NR) dye as a spectral probe by UV-vis absorption, fluorescence, and circular dichroism (CD) spectroscopy, as well as viscosity measurements and DNA melting techniques. It was found that farrerol molecules could intercalate into the base pairs of DNA as evidenced by decreases in iodide quenching effect and single-stranded DNA (ssDNA) quenching effect, induced CD spectral changes, and significant increases in relative viscosity and denaturation temperature of DNA. Furthermore, the spectral data matrix of the competitive reaction between farrerol and NR with DNA was resolved with an alternative least-squares (ALS) algorithm, and the concentration profiles in the reaction and the corresponding pure spectra for three species (farrerol, NR, and DNA-NR complex) were obtained. This ALS analysis demonstrated the intercalation of farrerol to the DNA by substituting for NR in the DNA-NR. complex. Moreover, the thermodynamic parameters enthalpy change (Delta H degrees) and entropy change (Delta S degrees) were calculated to be -16.49 +/- 051 kJ mol(-1) and 32.47 +/- 1.02 J mol(-1) K-1 via the van't Hoff equation, which suggested that the binding of farrerol to DNA was driven mainly by hydrophobic interactions and hydrogen bonds.