Increased expression of aldehyde dehydrogenase 1 A1 in nasopharyngeal carcinoma is associated with enhanced invasiveness
Increased expression of aldehyde dehydrogenase 1 A1 in nasopharyngeal carcinoma is associated with enhanced invasiveness
复制标题
DOI：
10.1007/s00405-013-2517-x
复制
发表时间：
2014-01-01
影响因子：
2.6
通讯作者：
Wen, Weiping
中科院分区：
文献类型：
--
作者：
Hou, Weijian;He, Weiling;Wen, Weiping
醛脱氢酶1 （ALDH1）的表达增加最近被报道在几种癌症中。然而，醛脱氢酶1 （ALDH1A1）成员A1是否参与鼻咽癌（NPC）的形成尚不清楚。为了研究ALDH1A1在鼻咽癌组织中的表达及其与鼻咽癌发生的关系，我们采用免疫组织化学（IHC）、定量RT-PCR （qRT-PCR）和Western blot检测了ALDH1A1在鼻咽癌组织中的表达。此外，我们利用流式细胞术对耳鼻癌细胞系CNE-2中ALDH1A1（高）细胞进行了筛选，并检测了原始胚胎干细胞标志物OCT4、SOX2和Nanog的表达。最后，我们在体外和体内研究了ALDH1A1（high）细胞的生长、增殖、集落形成和肿瘤发生能力。通过免疫组化、qRT-PCR和Western blot检测，我们发现ALDH1A1在人鼻咽癌中表达水平显著升高（p < 0.05）。流式细胞术从鼻鼻癌细胞系CNE-2中分选的ALDH1A1（高）细胞较ALDH1A1（低）细胞具有更高的原始胚胎干细胞标志物OCT4、SOX2和Nanog的表达，体外和体内的生长、增殖、集落形成和肿瘤发生能力均增强（p < 0.05）。我们的研究结果表明，ALDH1A1在鼻咽癌中的表达增加与侵袭性增强有关。
Increased expression of aldehyde dehydrogenase 1 (ALDH1) has recently been reported in several cancers. However, whether member A1 of aldehyde dehydrogenase 1 (ALDH1A1) is involved in the formation of nasopharyngeal carcinoma (NPC) remains unknown. To investigate the expression of ALDH1A1 in NPC and its association with the tumorigenesis of NPC, we examined the expression of ALDH1A1 in NPC specimens using immunohistochemistry (IHC), quantitative RT-PCR (qRT-PCR) and Western blot. Moreover, we sorted ALDH1A1(high) cells from NPC cell line CNE-2 by flow cytometry and examined the expression of primitive embryonic stem cell markers OCT4, SOX2 and Nanog. Finally, we investigated the capacities of growth, proliferation, colony- formation and tumorigenesis of ALDH1A1(high) cells in vitro and in vivo. We found ALDH1A1 was significantly increased in human NPC samples via IHC, qRT-PCR and Western blot (p < 0.05). ALDH1A1(high) cells sorted from NPC cell line CNE-2 by flow cytometry had higher expression of primitive embryonic stem cell markers OCT4, SOX2 and Nanog, and showed enhanced capacities of growth, proliferation, colony formation and tumorigenesis in vitro and in vivo when compared with ALDH1A1(low) cells (p < 0.05). Our findings indicated that increased expression of ALDH1A1 in NPC was associated with enhanced invasiveness.