Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?
Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?
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DOI：
10.1107/s0907444905033627
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发表时间：
2006-02-01
影响因子：
2.2
通讯作者：
McSweeney, SM
中科院分区：
文献类型：
--
作者：
Leiros, HKS;Timmins, J;McSweeney, SM
This paper focuses on the radiation-damage effects when applying the same total X-ray dose to protein crystals at different dose rates. These experiments have been performed on both a selenomethionated protein and on bovine trypsin using dose rates that span nearly two orders of magnitude. The results show no clear dose-rate effect on the global indicators of radiation damage, but a small measurable dose-rate effect could be found when studying specific radiation damage. It is hypothesized that this observed dose-rate effect relates to differences in the steady-state free-radical concentration.
