Microliter Operation for Determination of Nitrate-Nitrogen via Simple Zinc Reduction and Color Formation in a Well Plate with a Smartphone
Microliter Operation for Determination of Nitrate-Nitrogen via Simple Zinc Reduction and Color Formation in a Well Plate with a Smartphone
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DOI：
10.2116/analsci.18p497
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发表时间：
2019-04-01
影响因子：
1.6
通讯作者：
Grudpan, Kate
中科院分区：
文献类型：
--
作者：
Jaikang, Pheeraya;Wangkarn, Sununta;Grudpan, Kate
我们提出了一种简单的绿色比色方法，通过在 96 孔微孔板上操作并使用智能手机摄像头作为简单检测器来测定硝酸盐氮。使用含有0.3 mg锌的浆液将硝酸盐还原为亚硝酸盐，将还原液转移至96孔微孔板中与Griess试剂反应，形成粉红色偶氮染料产物。彩色产品图像分别由智能手机相机和 ImageJ 软件捕获和处理。智能手机摄像头的硝酸盐氮检测限和定量限分别为 0.04 和 0.10 mg/L。演示了对实际样品的应用。所提出的方法结果与肼还原法没有显着差异（95% 置信度）。该方法具有便携性和快速性等优点，可以作为现场分析的替代方法；可在 12 分钟内同时进行 20 个样品的重复运行。
We propose a simple greener colorimetric method for the determination of nitrate-nitrogen by operating on a 96-well microplate and using a smartphone camera as a simple detector. A slurry containing 0.3 mg zinc was used for reduction of nitrate to nitrite, the reduction solution was transferred to a 96-well microplate to react with Griess reagent to form a pink azo dye product. The color product image was captured and processed by a smartphone camera and ImageJ software, respectively. The limit of detection and limit of quantitation were 0.04 and 0.10 mg/L nitrate-nitrogen, respectively, for the smartphone camera. Application to real samples was demonstrated. The proposed method results showed no significant difference (at 95% confidence) with the hydrazine reduction method. The proposed method could be used as an alternative method for on-site analysis due to the advantages of portability and rapidity; duplicate run of 20 samples could be carried out simultaneously in 12 min.