Nonepidermal members of the keratin multigene family: cDNA sequences and in situ localization of the mRNAs.
Nonepidermal members of the keratin multigene family: cDNA sequences and in situ localization of the mRNAs.
复制标题
角蛋白多基因家族的非表皮成员：cDNA 序列和 mRNA 的原位定位。
DOI：
10.1093/nar/14.2.751
复制
发表时间：
1986
影响因子：
14.9
通讯作者：
H. Winter
中科院分区：
文献类型：
--
作者：
B. Knapp;M. Rentrop;J. Schweizer;H. Winter
由I型47kd和II型57kd蛋白组成的角蛋白集合作为小鼠各种内部复层上皮的角蛋白模式的主要成分而出现。我们从小鼠舌上皮polyA+RNA构建的cDNA文库中分离出两个互补角蛋白亚基的特异性cDNA克隆，并给出了57kd蛋白的完整核苷酸序列和推导的氨基酸序列，以及47kd蛋白的约75%的相应数据。编码两种类型角蛋白的已知表皮角蛋白mRNA的序列数据的比较揭示了mRNA的高度保守和可变结构域的基本相同和类型特异性组织。为了避免与角蛋白多基因家族的其他成员发生交叉反应，使用适当泰勒化的35S标记的cDNA探针与组织切片进行原位杂交，所述探针包含mRNA的低和非同源的3'编码和非编码结构域。相应的转录本的定位和分布表明，在小鼠舌上皮细胞的角蛋白表达强烈的区室化。
A keratin set which consists of a type I 47kd and a type II 57kd protein occurs as a major constituent of the keratin patterns of various internal stratified epithelia of the mouse. We have isolated specific cDNA clones of the two complementary keratin subunits from a cDNA library constructed with polyA+RNA of mouse tongue epithelium and present the complete nucleotide and deduced amino acid sequences of the 57kd protein and about 75% of the corresponding data of the 47kd protein. The comparison of the sequence data with those of known epidermal keratin mRNAs coding for the two types of keratin proteins reveals a fundamentally identical and type-specific organization of the mRNAs into both highly conserved and variable domains. In order to avoid cross-reactions with other members of the keratin multigene family, appropriately taylored 35S-labeled cDNA probes comprising the low and non-homologous 3' coding and noncoding domains of the mRNAs were used for in situ hybridization to tissue sections. The localization and distribution of the corresponding transcripts indicates a strongly compartmentalized keratin expression in mouse tongue epithelium.