Marine collagen-chitosan-fucoidan cryogels as cell-laden biocomposites envisaging tissue engineering
Marine collagen-chitosan-fucoidan cryogels as cell-laden biocomposites envisaging tissue engineering
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DOI：
10.1088/1748-605x/ab9f04
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发表时间：
2020-09-01
影响因子：
4
通讯作者：
Silva, Tiago H.
中科院分区：
文献类型：
--
作者：
Carvalho, Duarte Nuno;Lopez-Cebral, Rita;Silva, Tiago H.
由于海洋生物聚合物与存在于不同人体组织的细胞外基质中的蛋白质和多糖的相似性，其在组织工程（TE）应用中的结合引起了人们的高度关注。这篇论文报道了创新胶原蛋白-壳聚糖-岩藻聚糖冷冻形成的同时混合这三种海洋聚合物在无化学交联的方法。通过FTIR、氨基酸分析、圆二色性和SDS-PAGE等对海洋生物聚合物进行了理化表征，结果表明水母胶原未变性（保留了三螺旋结构），与II型胶原具有相似性。壳聚糖脱乙酰度高（90.1%），对高分子的理化性质和生物材料的形成有重要影响。反过来，流变学和扫描电镜研究证实，这些新型冷冻液在TE用途上具有有趣的特性，例如有效混合生物聚合物而没有明显的材料分离，机械稳定性（强粘弹性），以及足够的孔隙度来支持细胞增殖和营养物质和废物的交换。此外，在所有冷冻凝胶配方的体外细胞评估显示非细胞毒性行为。MTS实验、活/死实验和细胞形态评价（phalloidin DAPI）结果表明，低温环境可为细胞培养提供适宜的微环境，支持细胞活力，促进细胞增殖。总的来说，所获得的结果表明，本文提出的新型胶原-壳聚糖-岩藻聚糖冷冻材料是展望组织工程用途的有前途的支架，既可以作为非细胞生物材料，也可以作为细胞装载的冷冻材料。
The combination of marine origin biopolymers for tissue engineering (TE) applications is of high interest, due to their similarities with the proteins and polysaccharides present in the extracellular matrix of different human tissues. This manuscript reports on innovative collagen-chitosan-fucoidan cryogels formed by the simultaneous blending of these three marine polymers in a chemical-free crosslinking approach. The physicochemical characterization of marine biopolymers comprised FTIR, amino acid analysis, circular dichroism and SDS-PAGE, and suggested that the jellyfish collagen used in the cryogels was not denatured (preserved the triple helical structure) and had similarities with type II collagen. The chitosan presented a high deacetylation degree (90.1%) that can strongly influence the polymer physicochemical properties and biomaterial formation. By its turn, rheology, and SEM studies confirmed that these novel cryogels present interesting properties for TE purposes, such as effective blending of biopolymers without visible material segregation, mechanical stability (strong viscoelastic character), as well as adequate porosity to support cell proliferation and exchange of nutrients and waste products. Additionally,in vitrocellular assessments of all cryogel formulations revealed a non-cytotoxic behavior. The MTS test, live/dead assay and cell morphology assessment (phalloidin DAPI) showed that cryogels can provide a proper microenvironment for cell culturing, supporting cell viability and promoting cell proliferation. Overall, the obtained results suggest that the novel collagen-chitosan-fucoidan cryogels herein presented are promising scaffolds envisaging tissue engineering purposes, as both acellular biomaterials or cell-laden cryogels.