Expression of hornerin in stratified squamous epithelium in the mouse: A comparative analysis with profilaggrin
Expression of hornerin in stratified squamous epithelium in the mouse: A comparative analysis with profilaggrin
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DOI：
10.1177/002215540305100410
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发表时间：
2003-04-01
影响因子：
3.2
通讯作者：
Huh, NH
中科院分区：
文献类型：
--
作者：
Makino, T;Takaishi, M;Huh, NH
我们最近发现了一种新的蛋白质命名为hornerin，其结构特征是最相似的原丝蛋白，表皮组织角化的必需蛋白。在这项研究中，我们研究了角蛋白的表达相比，在各种小鼠组织中的聚丝蛋白原。Hornerin在新生小鼠皮肤的上表皮中表达，就像聚丝蛋白原一样。此外，角蛋白和聚丝蛋白原在舌、食管和前胃中表达。在所有四种组织中，角蛋白和原丝聚集蛋白的免疫染色均显示出颗粒状模式，并且这两种蛋白质的大部分信号共定位在透明角质蛋白颗粒上。这是通过双重免疫电子显微镜证实。在透明角质颗粒内，角蛋白更频繁地在外周中检测到，而聚丝蛋白原均匀分布。定量RT-PCR显示，这两种基因在前胃中的表达水平最高，在皮肤中的表达水平次之。前丝聚蛋白mRNA在前胃中最丰富。在皮肤中，角蛋白mRNA的量比聚丝蛋白原mRNA的量大四倍以上。这些结果形成的基础上，更好地了解可能的重叠和/或差异功能的角蛋白和聚丝蛋白原。
We have recently identified a novel protein named hornerin, the structural features of which are most similar to those of profilaggrin, an essential protein for keratinization of epidermal tissues. In this study we examined the expression of hornerin compared with that of profilaggrin in various mouse tissues. Hornerin was expressed in the upper epidermis of newborn mouse skin, as was profilaggrin. In addition, both hornerin and profilaggrin were expressed in the tongue, esophagus, and forestomach. In all four tissues, immunostaining for hornerin and profilaggrin showed a granular pattern, and most of the signals for the two proteins were co-localized on keratohyalin granules. This was confirmed by double immunoelectron microscopy. Within keratohyalin granules, hornerin was detected more frequently in the periphery, whereas profilaggrin was equally distributed. A quantitative RT-PCR revealed that both genes were expressed at highest levels in the forestomach and at the next highest levels in skin. Profilaggrin mRNA was most abundant in the forestomach. In skin, the amount of hornerin mRNA was more than fourfold greater than the amount of profilaggrin mRNA. These results form the basis for a better understanding of possible overlapping and/or differential functions of hornerin and profilaggrin.