Single ingestion of soy β-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects.
Single ingestion of soy β-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects.
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DOI：
10.1038/srep28183
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发表时间：
2016-06-17
影响因子：
4.6
通讯作者：
Sato R
中科院分区：
文献类型：
--
作者：
Hashidume T;Kato A;Tanaka T;Miyoshi S;Itoh N;Nakata R;Inoue H;Oikawa A;Nakai Y;Shimizu M;Inoue J;Sato R
大豆蛋白β-伴大豆球蛋白具有降血脂和抗肥胖作用。我们发现，禁食后单次摄入β-伴大豆球蛋白改变了小鼠肝脏中的基因表达。成纤维细胞生长因子21（FGF 21）基因表达的急剧增加，这是由正常喂养抑制，导致餐后循环FGF 21水平增加沿着脂肪组织重量的显着减少。大多数基因表达的增加，包括FGF 21，是转录激活因子4（ATF 4）的靶点，而不是过氧化物酶体增殖物激活受体α。ATF 4的显性负性形式的过表达显著降低β-伴大豆球蛋白诱导的肝FGF 21基因表达的增加。在FGF 21缺陷型小鼠中，β-伴大豆球蛋白的作用被部分消除。向饮食或原代肝细胞培养基中补充蛋氨酸证明了其对激活肝脏或肝细胞ATF 4-FGF 21信号传导的重要性。因此，膳食β-伴大豆球蛋白摄入可通过增加餐后循环FGF 21水平来影响肝脏和全身代谢。
Soy protein β-conglycinin has serum lipid-lowering and anti-obesity effects. We showed that single ingestion of β-conglycinin after fasting alters gene expression in mouse liver. A sharp increase in fibroblast growth factor 21 (FGF21) gene expression, which is depressed by normal feeding, resulted in increased postprandial circulating FGF21 levels along with a significant decrease in adipose tissue weights. Most increases in gene expressions, including FGF21, were targets for the activating transcription factor 4 (ATF4), but not for peroxisome proliferator-activated receptor α. Overexpression of a dominant-negative form of ATF4 significantly reduced β-conglycinin-induced increases in hepatic FGF21 gene expression. In FGF21-deficient mice, β-conglycinin effects were partially abolished. Methionine supplementation to the diet or primary hepatocyte culture medium demonstrated its importance for activating liver or hepatocyte ATF4-FGF21 signaling. Thus, dietary β-conglycinin intake can impact hepatic and systemic metabolism by increasing the postprandial circulating FGF21 levels.