Tanshinone IIA affects the HDL subfractions distribution not serum lipid levels: Involving in intake and efflux of cholesterol
Tanshinone IIA affects the HDL subfractions distribution not serum lipid levels: Involving in intake and efflux of cholesterol
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丹参酮 IIA 影响 HDL 亚组分分布而不是血清脂质水平：参与胆固醇的摄入和流出
DOI：
10.1016/j.abb.2016.01.001
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发表时间：
2016-02-15
影响因子：
3.9
通讯作者：
Yang, Guan-lin
中科院分区：
文献类型：
--
作者：
Jia, Lian-qun;Zhang, Ni;Yang, Guan-lin
研究目的：丹参酮IIA是中药的活性成分。本研究旨在探讨丹参酮IIA抗动脉粥样硬化的作用机制，可能是由于丹参酮IIA能够影响HDL亚组分的分布，进而调节胆固醇的逆向转运。 材料与方法：采用大鼠高脂血症模型。高脂血症后每日给予丹参酮IIA。体内分析肝脏脂质沉积和形态变化；测量血清和肝脏中的 HDL 亚组分和脂质水平；评估了与肝脏中胆固醇摄入和腹膜巨噬细胞胆固醇流出相关的基因的表达。在体外，用丹参酮IIA预处理HepG2细胞和THP-1细胞，然后用ox-LDL预处理，以评估总胆固醇和相关基因的表达。结果：丹参酮IIA减少了肝脏中的脂质沉积。此外，它不影响血脂水平，但降低了HDL中亚组分的水平，并升高了HDL大亚组分的水平。此外，丹参酮IIA还可以调节肝脏中CYP7A1、LDL-R、SREBP2和LCAT的表达以及巨噬细胞中分别参与胆固醇摄入和流出的ABCA1和CD36的表达。它可以减少ox-LDL诱导引起的脂质积累，并调节HepG2中LDL-R、HMGCR和SREBP2以及THP-1细胞中ABCA1、CD36的表达。结论：丹参酮IIA不降低血脂水平，而是影响HDL亚组分分布，从而调节胆固醇的摄入和流出。 (C) 2016 Elsevier Inc. 保留所有权利。
Aim of study: Tanshinone IIA is an active component of the traditional Chinese medicine. This study aimed at investigating the mechanism of tanshinone IIA on anti-atherosclerosis, which may be because of that Tanshinone IIA can affect the HDL subfractions distribution and then regulate reverse cholesterol transport.Materials and methods: A model of hyperlipidaemia in rats was used. Tanshinone IIA was given daily after hyperlipidaemia. In vivo, lipid deposition and morphological changes in liver were analyzed; HDL sub fractions and lipid level in serum as well as in liver were measured; the expression of genes related to cholesterol intake in liver and peritoneal macrophage cholesterol efflux were evaluated. In vitro, HepG2 cells and THP-1 cells were pretreated with tanshinone IIA and subsequently with ox-LDL to evaluate the total cholesterol and the expression of related genes.Results: Tanshinone IIA reduced the lipid deposition in liver. Moreover, it did not affect the serum lipid levels but reduced the levels of HDL middle subfractions and increased the levels of HDL large sub fractions. Furthermore, tanshinone IIA could regulate the expressions of CYP7A1, LDL-R, SREBP2 and LCAT in the liver as well as the ABCA1 and CD36 in macrophage cells which is involving in the cholesterol intake and efflux respectively. It could reduce lipid accumulation caused by ox-LDL induction, and that also regulate the expressions of LDL-R, HMGCR and SREBP2 in HepG2 and ABCA1, CD36 in THP-1 cells.Conclusion: A novel finding that tanshinone IIA was not reduce the serum lipid level but affects the HDL subfractions distribution and thereby regulating the intake and efflux of cholesterol. (C) 2016 Elsevier Inc. All rights reserved.