High-Throughput Analysis of Drug Safety Responses in Induced Pluripotent Stem Cell-Derived Cardiomyocytes Using Multielectrode Array.
High-Throughput Analysis of Drug Safety Responses in Induced Pluripotent Stem Cell-Derived Cardiomyocytes Using Multielectrode Array.
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使用多电极阵列对诱导多能干细胞来源的心肌细胞的药物安全反应进行高通量分析。
DOI：
10.1007/978-1-0716-2261-2_7
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Wu,JosephC
中科院分区：
文献类型：
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作者：
Belbachir,Nadjet;Cunningham,Nathan;Wu,JosephC
微电极阵列(MEA)是一种用于跟踪可兴奋细胞内离子通道活动的电生理仪器。神经元和心肌细胞被种植在一片能够检测电信号的传感器上形成细胞单层，称为细胞外场电位(EFP)。这种非侵入性工具使研究人员能够调查关键参数，如EFP幅度、持续时间和心律失常。MEA逐渐被认为是高通量体外电生理评估的金标准，特别是在心脏疾病建模和心脏毒性评估方面。
Microelectrode array (MEA) is an electrophysiological instrument used to track activities of ion channels in excitable cells. Neurons and cardiomyocytes are seeded to form a cell monolayer on a field of sensors able to detect electrical signals, called extracellular field potentials (EFPs). This noninvasive tool allows researchers to investigate key parameters such as EFP amplitude, duration, and arrhythmias. MEA is progressively considered the gold standard for high-throughput in vitro electrophysiological evaluation, particularly for cardiac disease modeling and cardiac toxicity assessment.