BRAIN-DERIVED NEUROTROPHIC FACTOR AND NEUROTROPHIN-3 SUPPORT THE SURVIVAL AND NEURITOGENESIS RESPONSE OF DEVELOPING COCHLEOVESTIBULAR GANGLION NEURONS
BRAIN-DERIVED NEUROTROPHIC FACTOR AND NEUROTROPHIN-3 SUPPORT THE SURVIVAL AND NEURITOGENESIS RESPONSE OF DEVELOPING COCHLEOVESTIBULAR GANGLION NEURONS
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DOI：
10.1006/dbio.1993.1239
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发表时间：
1993-09-01
影响因子：
2.7
通讯作者：
REPRESA, J
中科院分区：
文献类型：
--
作者：
AVILA, MA;VARELANIETO, I;REPRESA, J
研究了脑源性神经营养因子(BDNF)和神经营养素-3(NT-3)对禽类耳蜗前庭神经节分化的影响及其与糖基磷脂酰肌醇(GPI)水解酶的关系。BDNF和NT-3(2 ng/ml)促进耳蜗神经节和前庭神经节外植体突起生长。这种对神经发生的影响是阶段依赖的，NT-3在E7达到最大值，BDNF在E9达到最大。前庭神经节对脑源性神经营养因子的反应总是小于同阶段的耳蜗神经节。在分离的神经细胞培养中，BDNF和NT-3对神经元存活和轴突延伸也有刺激作用。BDNF和NT-3的浓度分别为4 ng/ml和2 ng/ml时，对鸡耳蜗神经节最敏感阶段的半数最大效应分别出现在2和1 ng/ml。肌醇磷酸多糖(IPG)不能模拟BDNF或NT-3对神经元存活和轴突生长的影响，也不能增强它们的反应。针对IPG的抗体并不能阻断这些神经营养因子的作用。提示BDNF和NT-3在内耳神经支配模式的建立中可能起协同作用。与早期的增殖作用不同，神经营养作用不依赖于GPI/IPG信号转导系统。
The effects of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) on the differentiation of avian cochleovestibular ganglion and their possible association with the hydrolysis of glycosyl-phosphatidylinositol (GPI) were studied. BDNF and NT-3 (2 ng/ml) promoted neurite outgrowth in explants of both cochlear and vestibular ganglia. This effect on neuritogenesis was stage-dependent, reaching a maximum at E7 for NT-3 and at E9 for BDNF. The magnitude of the response of the vestibular ganglion to BDNF was always smaller than that of the cochlear ganglion of an equivalent stage. BDNF and NT-3 stimulation of neuronal survival and neurite extension was also demonstrated in dissociated neuronal cell cultures. The effect was concentration-dependent with saturation of the response occurring at 4 ng/ml for BDNF and at 2 ng/ml for NT-3, the half-maximal effect occurring at 2 and 1 ng/ml, respectively, for the most sensitive stages of the chick cochlear ganglion. Inositol phosphoglycan (IPG) did not mimic the effects of BDNF or NT-3 on neuronal survival and neurite outgrowth, nor was it able to potentiate their responses. Antibodies raised against IPG did not block the effects of these neurotrophins. The results suggest that BDNF and NT-3 may act in cooperation to establish the innervation pattern of the inner ear. Unlike their early proliferative effects, neurotrophic effects are uncoupled from the GPI/IPG signal transduction system.