Drosophila valois encodes a divergent WD protein that is required for Vasa localization and Oskar protein accumulation
Drosophila valois encodes a divergent WD protein that is required for Vasa localization and Oskar protein accumulation
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DOI：
10.1242/dev.01590
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发表时间：
2005-02-01
期刊：
影响因子：
4.6
通讯作者：
Suter, B
中科院分区：
文献类型：
--
作者：
Cavey, M;Hijal, S;Suter, B
在对果蝇母系效应致死突变的初步筛查中，Valois(VLS)被确定为后发组基因。尽管早期对其进行了遗传鉴定，但到目前为止还没有在分子水平上对其进行表征。我们发现VLS编码一个发散的WD结构域蛋白，并且三个可用的EMS诱导的点突变导致VLS ORF中的过早停止密码子。我们已经产生了一个零等位基因，它的表型比EMS突变体更强。Vls(空)突变体表明，Vls(+)对于高水平的Oskar蛋白在卵子发生过程中积累、Oskar在卵子发生后期的正常后部定位以及Vasa蛋白在极质组装的整个过程中的后部定位是必需的。没有证据表明VLS依赖于后通路的上游因素，这表明Valois蛋白(VLS)在这一过程中起到了辅助因素的作用。根据VLS的结构、相似蛋白质在不同系统中的功能以及我们的表型分析，VLS可能通过促进不同分子之间的相互作用来促进后图案形成。
valois (vls) was identified as a posterior group gene in the initial screens for Drosophild maternal-effect lethal mutations. Despite its early genetic identification, it has not been characterized at the molecular level until now. We show that vls encodes a divergent WD domain protein and that the three available EMS-induced point mutations cause premature stop codons in the vls ORF. We have generated a null allele that has a stronger phenotype than the EMS mutants. The vls(null) mutant shows that vls(+) is required for high levels of Oskar protein to accumulate during oogenesis, for normal posterior localization of Oskar in later stages of oogenesis and for posterior localization of the Vasa protein during the entire process of pole plasm assembly. There is no evidence for vls being dependent on an upstream factor of the posterior pathway, suggesting that Valois protein (Vls) instead acts as a cofactor in the process. Based on the structure of Vls, the function of similar proteins in different systems and our phenotypic analysis, it seems likely that vls may promote posterior patterning by facilitating interactions between different molecules.