Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus
Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus
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DOI：
10.1046/j.1365-313x.2002.01297.x
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发表时间：
2002-05-01
期刊：
影响因子：
7.2
通讯作者：
Dinesh-Kumar, SP
中科院分区：
文献类型：
--
作者：
Liu, YL;Schiff, M;Dinesh-Kumar, SP
烟草N基因赋予对烟草花叶病毒（TMV）的抗性，并编码Toll-白细胞介素-1受体/核苷酸结合位点/富含亮氨酸重复序列（TIR-NBS-LRR）类蛋白。我们已经开发并使用了基于烟草脆裂病毒（TRV）的病毒诱导的基因沉默（VIGS）系统来研究烟草候选基因在氮介导的信号传导途径中的作用。为了实现这一点，我们产生了含有烟草N基因的转基因本氏烟草。转基因株系对TMV表现出过敏反应，并限制病毒向接种部位扩散。这表明烟草N基因在异源N中能赋予对TMV的抗性。本萨米亚纳我们已经使用该品系使用基于TRV的VIGS方法来研究烟草Rar 1-、EDS 1-和NPR 1/NIM 1- like基因在N介导的对TMV的抗性中的作用。我们的VIGS分析表明，这些基因是N功能所必需的。EDS 1样基因对N功能的需求表明，EDS 1可能是由TIR-NBS-LRR类抗性蛋白介导的细菌、真菌和病毒抗性信号传导的共同组分。氮介导的对TMV的抗性需要Rar 1- like基因和大麦中的一些抗白粉病基因提供了TIR-NBS-LRR和CC-NBS-LRR蛋白介导的抗病信号传导途径中的会聚点的第一个例子。如本文所述的基于TRV的VIGS方法来研究N -介导的抗性信号传导将不仅可用于分析抗病性信号传导途径，而且可用于分析遗传上难处理的植物系统中的其它信号传导途径。
The tobacco N gene confers resistance to tobacco mosaic virus (TMV) and encodes a Toll-interleukin-1 receptor/nucleotide binding site/leucine-rich repeat (TIR-NBS-LRR) class protein. We have developed and used a tobacco rattle virus (TRV) based virus induced gene silencing (VIGS) system to investigate the role of tobacco candidate genes in the N -mediated signalling pathway. To accomplish this we generated transgenic Nicotiana benthamiana containing the tobacco N gene. The transgenic lines exhibit hypersensitive response (HR) to TMV and restrict virus spread to the inoculated site. This demonstrates that the tobacco N gene can confer resistance to TMV in heterologous N. benthamiana . We have used this line to study the role of tobacco Rar1- , EDS1- , and NPR1/NIM1- like genes in N -mediated resistance to TMV using a TRV based VIGS approach. Our VIGS analysis suggests that these genes are required for N function. EDS1 -like gene requirement for the N function suggests that EDS1 could be a common component of bacterial, fungal and viral resistance signalling mediated by the TIR-NBS-LRR class of resistance proteins. Requirement of Rar1 - like gene for N -mediated resistance to TMV and some powdery mildew resistance genes in barley provide the first example of converging points in the disease resistance signalling pathways mediated by TIR-NBS-LRR and CC-NBS-LRR proteins. The TRV based VIGS approach as described here to study N -mediated resistance signalling will be useful for the analysis of not only disease resistance signalling pathways but also of other signalling pathways in genetically intractable plant systems.