Lipopolysaccharide from Prevotella nigrescens stimulates osteoclastogenesis in cocultures of bone marrow mononuclear cells and primary osteoblasts
Lipopolysaccharide from Prevotella nigrescens stimulates osteoclastogenesis in cocultures of bone marrow mononuclear cells and primary osteoblasts
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DOI：
10.1111/j.1600-0765.2006.00876.x
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发表时间：
2006-08-01
影响因子：
3.5
通讯作者：
Ko, J. S.
中科院分区：
文献类型：
--
作者：
Chung, Y. -H.;Chang, E. -J.;Ko, J. S.
背景和目的：脂多糖被认为是与牙周病相关的病原体的主要毒力因子，并被认为可以刺激体内骨吸收。尽管黑普氏菌与牙周炎有关，但其在破骨细胞生成中的作用尚未见报道。在这项研究中，我们研究了来自P. nigrescens的脂多糖对破骨细胞形成和破骨细胞分化相关细胞因子产生的影响。材料和方法：在巨噬细胞集落刺激因子（M-CSF）和核因子κB配体受体激活剂（RANKL）存在下培养小鼠骨髓单核细胞，有或没有脂多糖。骨髓单核细胞也与颅骨成骨细胞在存在或不存在脂多糖的情况下共培养。通过抗酒石酸盐酸性磷酸酶细胞化学测定破骨细胞的形成。通过酶联免疫吸附测定 (ELISA) 测定骨保护素 (OPG)、M-CSF、肿瘤坏死因子 α (TNF-α)、转化生长因子 β (TGF-β) 和前列腺素 E-2 (PGE(2)) 的产生。结果：P. nigrescens 脂多糖抑制在 M-CSF 和 RANKL 存在下培养的骨髓单核细胞的破骨细胞分化。然而，在共培养系统中，P. nigrescens 脂多糖刺激破骨细胞生成。值得注意的是，P. nigrescens 脂多糖减少了 OPG 的产生，但增加了 TGF-β 的分泌。此外，用P. nigrescens 脂多糖处理可增加培养后期PGE(2) 的产量。 M-CSF 和 TNF-α 的产生没有差异。结论：这些结果表明，P. nigrescens 脂多糖通过减少 OPG 的产生并增加 TGF-β 和 PGE(2) 的产生来刺激共培养系统中的破骨细胞生成。通过这些因素的作用机制，黑曲霉脂多糖可能引起牙周病牙槽骨吸收。
Background and Objective: Lipopolysaccharide is thought to be a major virulence factor of pathogens associated with periodontal diseases and is believed to stimulate bone resorption in vivo. Although Prevotella nigrescens has been implicated in periodontitis, its role in osteoclastogenesis has not been reported. In this study, we investigated the effects of lipopolysaccharide from P. nigrescens on the formation of osteoclasts and the production of cytokines related to osteoclast differentiation.Material and Methods: Mouse bone marrow mononuclear cells were cultured in the presence of macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor kappa B ligand (RANKL), with or without lipopolysaccharide. Bone marrow mononuclear cells were also cocultured with calvarial osteoblastic cells in the presence or absence of lipopolysaccharide. Osteoclast formation was determined by tartrate-resistant acid phosphatase cytochemistry. The production of osteoprotegerin (OPG), M-CSF, tumor necrosis factor alpha (TNF-alpha), transforming growth factor-beta (TGF-beta) and prostaglandin E-2 (PGE(2)) was determined by enzyme-linked immunosorbent assay (ELISA).Results: P. nigrescens lipopolysaccharide inhibited osteoclast differentiation from bone marrow mononuclear cells cultured in the presence of M-CSF and RANKL. However, in the coculture system, P. nigrescens lipopolysaccharide stimulated osteoclastogenesis. Notably, P. nigrescens lipopolysaccharide decreased OPG production but increased TGF-beta secretion. In addition, treatment with P. nigrescens lipopolysaccharide increased PGE(2) production during the late stage of the culture period. There was no difference in M-CSF and TNF-alpha production.Conclusion: These results demonstrate that P. nigrescens lipopolysaccharide stimulates osteoclastogenesis in the coculture system by decreasing the production of OPG and increasing the production of TGF-beta and PGE(2). Through the mechanisms involving these factors, P. nigrescens lipopolysaccharide may cause alveolar bone resorption in periodontal diseases.