Co-occurrence of multiple plasmid-borne linezolid resistance genes-optrA, cfr, poxtA2 and cfr(D) in an Enterococcus faecalis isolate from retail meat.
Co-occurrence of multiple plasmid-borne linezolid resistance genes-optrA, cfr, poxtA2 and cfr(D) in an Enterococcus faecalis isolate from retail meat.
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DOI：
10.1093/jac/dkad142
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发表时间：
2023-05
期刊：
影响因子：
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通讯作者：
Weiyi Shen;Rong Zhang;Jiachang Cai
中科院分区：
文献类型：
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作者：
Weiyi Shen;Rong Zhang;Jiachang Cai
目的研究来自零售肉类的1株耐利奈唑胺粪肠球菌DM 86中质粒携带的利奈唑胺耐药基因optrA、cfr、poxtA 2和cfr（D）基因的分子特征和可转移性。方法E.通过PCR分析筛选粪肠球菌DM 86中是否存在已知的利奈唑胺耐药基因。利用接合试验研究了抗性基因的可转移性。E.使用Illumina和Nanopore平台两者获得粪肠球菌DM 86。结果E. faecalis DM 86属于序列类型116（ST 116）。在3种质粒上鉴定出4种利奈唑胺耐药基因，命名为pDM 86 -2-cfr、pDM 86 -3-optrA和pDM 86 -4-poxtA [cfr（D）共定位]。发现IS 1216移动的元件位于这两个质粒上的cfr和optrA基因座的侧翼。pDM 86 -3-optrA编码RDK型OptrA蛋白，并且在该质粒上鉴定了“IS 1216-fexA-optrA-pH 7A（A）-IS 1216”的共同遗传阵列。cfr（D）基因与pDM 86 -4-poxtA上的poxtA 2基因密切相关，最近在大肠杆菌中发现了类似的质粒和结构。动物源性粪便。研究了该质粒在大肠杆菌中的种内和种间水平转移性。粪肠球菌JH 2 -2、屎肠球菌BM 4105 RF和金黄色葡萄球菌RN 4220的检出率分别为2.8 × 10-3、1.7 × 10-3和3.4 × 10-5。结论这是首次报道在一株大肠杆菌中同时存在多达4个质粒携带的利奈唑胺耐药基因。粪便因此，应采取有效行动，以避免食品的微生物群污染和这些抗菌素耐药性储存库的进一步传播。
OBJECTIVES To investigate the molecular characteristics and transferability of plasmid-borne linezolid resistance genes optrA, cfr, poxtA2 and cfr(D) genes in one linezolid-resistant Enterococcus faecalis DM86 from retail meat. METHODS E. faecalis DM86 was screened for the presence of known linezolid resistance genes via PCR analysis. Conjugation experiments were used to evaluate the transferability of the resistance genes. The complete genome of E. faecalis DM86 was obtained using both the Illumina and Nanopore platforms. RESULTS Analysis of the complete sequence showed that E. faecalis DM86 belonged to sequence type 116 (ST116). Four linezolid resistance genes were identified on three plasmids, designated as pDM86-2-cfr, pDM86-3-optrA and pDM86-4-poxtA [cfr(D) co-located]. IS1216 mobile elements were found to flank the cfr and optrA locus on these two plasmids. pDM86-3-optrA encoded the RDK-type OptrA protein and a common genetic array of 'IS1216-fexA-optrA-erm(A)-IS1216' was identified on this plasmid. The cfr(D) gene was closely associated with the poxtA2 gene on pDM86-4-poxtA, and similar plasmids and structures were reported recently in the E. faecalis of animal origin. The intra- and inter-species horizontal transferability of this plasmid to E. faecalis JH2-2, Enterococcus faecium BM4105RF and Staphylococcus aureus RN4220 was also proved, with a frequency of 2.8 × 10-3, 1.7 × 10-3 and 3.4 × 10-5, respectively. CONCLUSIONS This was the first report of the co-existence of up to four plasmid-borne linezolid resistance genes in one E. faecalis. Thus, efficient actions should be exerted to circumvent the microbiota contamination of food and the further spread of these antimicrobial resistance reservoirs.