Comparative susceptibility of three species of Anopheles from Belize, Central America, to Plasmodium falciparum (NF-54)
Comparative susceptibility of three species of Anopheles from Belize, Central America, to Plasmodium falciparum (NF-54)
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DOI：
10.2987/8756-971x(2005)21
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发表时间：
2005-09-01
影响因子：
1
通讯作者：
Andre, RG
中科院分区：
文献类型：
--
作者：
Grieco, JP;Achee, NL;Andre, RG
2000年8月，对来自中美洲伯利兹的3种按蚊和实验室感染研究中使用的标准种斯氏按蚊进行了敏感性比较研究。受试人群通过膜饲养器喂养感染了恶性疟原虫(NF-54株)培养的人血。对照种为An.斯氏杆菌的感染率最高，73.8%的解剖标本在唾液腺中子孢子阳性。对照种的子孢子载量也较高，74.0%的阳性腺体子孢子数大于200。在来自伯利兹的物种中，达令按蚊是最敏感的物种，例如，41.0%的唾液腺呈阳性，每个腺体有200个以上的子孢子。前庭按蚊唾液腺感染率较低(9.3%)，腺体内有中等数量的子孢子(85.7%，含50~250个子孢子)。白纹伊蚊是最不容易感染的物种。没有An的标本。来自Golden Stream种群的白纹伊蚊在唾液腺中发育了子孢子，但20.7%的解剖标本中肠感染阳性。那就是安。白纹伊蚊种群也有类似的结果，唾液腺感染率仅为2.2%，中肠感染率为21.5%。卵囊内有卵囊。斯氏沼虾在第10天后体积增加了20%，第12天发育达到高峰，卵囊分化开始时卵囊平均直径为58微米。卵囊发育较慢。第10天以后，卵囊发育比前10天增加了53%。直到饲喂后第13天才观察到卵囊分化。就像一个。兽医，这两个种群都是。白纹伊蚊的卵囊发育速度较慢，但在第10天后没有明显的增长。金流种群的卵囊在第10天后停止生长，平均30亩m时达到峰值。生长一直持续到第13天和第14天(分别为36.7和35.7亩)。
In August of 2000, a comparative susceptibility study was conducted using 3 species of Anopheles mosquitoes from Belize, Central America, and a standard species used in laboratory infection studies, Anopheles stephensi. Test populations were fed human blood infected with cultured Plasmodium falciparum (NF-54 strain) parasites via a membrane feeder. The control species, An. stephensi, exhibited the highest infections, with 73.8% of dissected specimens positive for sporozoites in the salivary glands. The control species also showed heavier sporozoite loads; 74.0% of positive glands having greater than 200 sporozoites. Of species from Belize, Anopheles darlingi was the most susceptibile, e.g., 41.0% of salivary glands were positive, with more than 200 sporozoites per gland. Anopheles vestitipennis had a low salivary gland infection rate (9.3%) and a moderate number of sporozoites in glands (i.e., 85.7% containing 50-250 sporozoites). Anopheles albimanus was the least susceptible species to infection. No specimens of An. albimanus from the Golden Stream population developed sporozoites in the salivary glands, yet 20.7% of dissected specimens had positive midgut infections. The An. albimanus Buena Vista population showed similar results with only a 2.2% salivary gland infection rate and a 21.5% midgut infection rate. Oocysts in An. stephensi increased in size by 20% after day 10. Development peaked at day 12, with a mean oocyst diameter of 58 mu m at onset of oocyst differentiation. Oocysts developed more slowly in An. vestitipennis until day 10. After day 10, there was a 53% increase in oocyst development over the previous 10 days. Oocyst differentiation was not observed until day 13 postfeed. As with An. vestitipennis, both populations of An. albimanus showed similar slow rates of oocyst development; however, no dramatic growth increase occurred after day 10. The oocysts in the Golden Stream population exhibited a cessation of growth after day 10, peaking at a mean of 30 mu m. The Buena Vista population did not exhibit the same level of reduced oocyst development. A gradual increase in growth continued until days 13 and 14 (36.7 and 35.7 mu m, respectively).